Virus causing respiratory tract illness in susceptible mammals
The invention relates to the field of virology. The invention provides an isolated essentially mammalian negative-sense single stranded RNA virus (MPV) within the subfamily Pneumovirinae of the family Paramyxoviridae and identifiable as phylogenetically corresponding to the genus Metapneumovirus and components thereof.
1. A method for detecting a human metapneumovirus in a sample, wherein the method comprises contacting the sample with a nucleic acid encoding an amino acid sequence that is greater than 88% identical to the amino acid sequence of the N protein of MPV isolate 00-1, SEQ ID NO.: 1.
2. The method of claim 1 , wherein the amino acid sequence is: SEQ ID No.: 1.
3. A method for detecting a human metapneumovirus in a sample, wherein the method comprises contacting the sample with an antibody that specifically binds to a protein that is greater than 88% identical to the amino acid sequence of the N protein of MPV isolate 00-1, SEQ ID NO.: 1.
4. The method of claim 3 , wherein the protein consists of an amino acid sequence of SEQ ID No.: 1.
5. The method of claim 4 , wherein the method further comprises an immune fluorescence assay.
6. A method for detecting a human metapneumovirus in a sample, wherein the method comprises contacting the sample with a first nucleic acid that is at least 90% homologous to a second nucleic acid, wherein the second nucleic acid encodes, or wherein the second nucleic acid is complementary to the nucleic acid that encodes, a protein, or fragment thereof, consisting of a sequence that is greater than 88% identical to the amino acid sequence of the N protein of MPV isolate 00-1, SEQ ID NO.: 1.
7. A method for detecting a human metapneumovirus in a sample, wherein the method comprises contacting the sample with one or more nucleic acids that are at least 90% homologous to the genome or antigenome of the virus isolate deposited as I-2614 with CNCM, Paris.
8. The method of claim 1 or 7 , wherein the nucleic acid sequence is at least 90% identical to SEQ ID No.: 1.
9. A method for detecting an antibody against human metapneumovirus in a sample, wherein the method comprises contacting the sample with a protein comprising the amino acid sequence of: SEQ ID No.: 1.