Polymorphous forms of rifaximin, processes for their production and use thereof in medicinal preparations
View Patent ↗Crystalline polymorphous forms of the rifaximin (INN) antibiotic named rifaximin α and rifaximin β, and a poorly crystalline form named rifaximin γ have been discovered. These forms are useful in the production of medicinal preparations for oral and topical use and can be obtained by means of a crystallization process carried out by hot-dissolving the raw rifaximin in ethyl alcohol and by causing the crystallization of the product by the addition of water at a determinate temperature and for a determinate period of time. The crystallization is followed by drying carried out under controlled conditions until a specific water content is reached in the end product.
1. A purified rifaximin α, a polymorph of the antibiotic rifaximin, wherein said rifaximin α has a water content of 3% or less, and produces a powder X-ray diffractogram showing peaks at values of the diffraction angles 2θ of 6.6°; 7.4°; 7.9°; 8.8°; 10.5°; 11.1°; 11.8°; 12.9°; 17.6°; 18.5°; 19.7°; 21.0°; 21.4°; 22.1°.
2. The rifaximin α according to claim 1 , wherein said water content is between 2.0% and 3.0%.
3. A purified rifaximin β, a polymorph of the antibiotic rifaximin wherein said rifaximin β has a water content higher than 4.5% and produces a powder X-ray diffractogram showing peaks at values of the diffraction angles 2θ of 5.4°; 6.4°; 7.0°; 7.8°; 9.0°; 10.4°; 13.1°, 14.4°; 17.1°; 17.9°; 18.3°; 20.9°.
4. The rifaximin β according to claim 3 , wherein said water content is between 5.0% and 6.0%.
5. A purified rifaximin γ, a polymorph of the antibiotic rifaximin wherein said rifaximin γ has a water content between 1.0% and 2.0% and produces a powder X-ray diffractogram showing a mainly amorphous profile and few significant peaks at values of diffraction angles 2θ of 5.0°; 7.1°; 8.4°.
6. A process for the production of rifaximins α, β and γ, comprising:
reacting a molar equivalent of rifamycin O with an excess of 2-amino-4-methylpyridine in a solvent mixture of water and ethyl alcohol in a volumetric ratio between 1:1 and 2:1, for a period of time between 2 and 8 hours, at a temperature between 40° C. and 60° C.,
treating the reaction mass at room temperature with a solution of ascorbic acid in a mixture of water, ethyl alcohol and concentrated aqueous hydrochloric acid,
adjusting the pH of the reaction mass to pH 2.0 with a concentrated aqueous solution of hydrochloric acid,
filtering the suspension,
washing any resulting solid with the water/ethyl alcohol solvent mixture to obtain raw rifaximin,
purifying the raw rifaximin by dissolving it in ethyl alcohol at a temperature between 45° C. and 65° C.,
precipitating the raw rifaximin by adding water and by lowering the temperature of the suspension to between 0° C. to 50° C. under stirring for a period of time between 4 and 36 hours,
filtering the suspension,
washing the resulting solid with water, and
drying it under vacuum or under conditions of normal pressure, with or without a drying agent, at a temperature between room temperature and 105° C., for a period of time between 2 and 72 hours to the water content required to form rifaximin α, β or γ.
7. The process according to claim 6 , wherein said 2-amino-4-methylpyridine is from 2.0 to 3.5 molar equivalents.
8. The process according to claim 6 , wherein said water added to precipitate the raw rifaximin is in a weight amount between 15% and 70% with respect to the weight amount of ethyl alcohol used for the dissolution.
9. The process according to claim 6 for the production of rifaximin α, wherein after the addition of water to the raw rifaximin, the temperature is lowered to a value between 28° C. and 32° C. in order to cause the beginning of the crystallization,
stirring the resulting suspension at a temperature between 40° C. and 50° C. for a period of time between 6 and 24 hours,
cooling the suspension to 0° C. for a period of time between 15 minutes and one hour,
filtering the suspension, washing the resulting solid with water, and
drying the washed solid until a water content lower than 4.5% is reached.
10. The process according to claim 9 , wherein said water content is between 2.0% and 3.0%.
11. The process according to claim 6 for the production of rifaximin β,
wherein after the addition of water to the raw rifaximin, the temperature is lowered to a value between 28° C. and 32° C. in order to cause the beginning of the crystallization,
stirring the resulting suspension at a temperature between 40° C. and 50° C. for a period of time between 6 and 24 hours,
cooling the suspension to 0° C. for a period of time between 15 minutes and one hour,
filtering the suspension, washing the resulting solid with water, and drying the washed solid until a water content higher than 4.5% is reached.
12. The process according to claim 11 , wherein said water content is between 5.0% and 6.0%.
13. The process according to claim 6 for the production of rifaximin γ, wherein after the addition of water to the raw rifaximin, the temperature is lowered to a value between 28° C. and 32° C. in order to cause the beginning of the crystallization,
cooling the suspension to 0° C. for a period of time between 6 and 24 hours,
filtering the suspension, washing the resulting solid with water and
drying the washed solid until a water content between 1.0% and 2.0% is reached.
14. A process for the production of rifaximin α, comprising suspending rifaximin γ in a solvent mixture of ethyl alcohol/water in a volumetric ratio of 7:3,
heating the suspension at a temperature between 38° C. and 50° C., under stirring, for a period of time between 6 and 36 hours,
filtering the suspension,
washing the resulting solid with water, and
drying the washed solid until a water content lower than 4.5% is reached.
15. The process according to claim 14 , wherein said water content is between 2.0% and 3.0%.
16. A process for the production of rifaximin β, comprising
suspending rifaximin γ in a solvent mixture of ethyl alcohol/water in a volumetric ratio of 7:3,
heating the suspension at a temperature between 38° C. and 50° C., under stirring, for a period of time between 6 and 36 hours,
filtering the suspension,
washing the resulting solid with water, and
drying the washed solid until a water content higher than 4.5% is reached.
17. The process according to claim 16 , wherein said water content is between 5.0% and 6.0%.
18. A process for the production of rifaximin γ, comprising
dissolving rifaximin α or β in ethyl alcohol at a temperature between 50° C. and 60° C.,
adding demineralized water until an ethyl alcohol/water volumetric ratio equal to 7:3 is reached,
cooling the solution to 30° C. under strong stirring,
further cooling the resulting suspension to 0° C. for a period of time between 6 and 24 hours,
filtering said suspension,
washing the resulting solid with water, and
drying the solid until a water content lower than 2.0% is reached.
19. A process for the production of rifaximin β, comprising keeping rifaximin α in an ambient environment having a relative humidity higher than 50% for a period of time between 12 and 48 hours until said rifaximin α is converted into rifaximin β.
20. A process for the production of rifaximin α, comprising drying rifaximin β under atmospheric pressure, or under vacuum, or in the presence of a drying agent, at a temperature between the room temperature and 105° C., for a period of time between 2 and 72 hours until said rifaximin β is converted into rifaximin α.