Method for making medical devices having antimicrobial coatings thereon
View Patent ↗The present invention provides a method for preparing a medical device, preferably a contact lens, having an antimicrobial metal-containing LbL coating on a medical device, wherein the antimicrobial metal-containing LbL coating comprises at least one layer of a negatively charged polyionic material having —COOAg groups and/or silver nanoparticles formed by reducing Ag + ions associated with the —COO − groups of the negatively charged polyionic material. In addition, the present invention provides a medical device prepared according to a method of the invention.
1. A method for preparing a medical device having a silver nano-particle-containing antimicrobial LbL coating thereon, comprising the steps of:
(a) obtaining a medical device with a polyelectrolyte LbL coating thereon, wherein the polyelectrolyte LbL coating includes one or more bilayers of a negatively charged polyionic material having —COOH groups and a positively charged polyionic material;
(b) immersing the medical device having the polyelectrolyte LbL coating in a solution containing silver ions for a period of time sufficient to replace a desired amount of H + with silver ions; and
(c) autoclaving the medical device of step (b) to reduce the silver ions contained in the polyelectrolyte LbL coating to form silver nano-particles wherein the medical device having the silver nano-particle-containing antimicrobial LbL coating exhibits at least 50% inhibition of viable microorganisms.
2. The method of claim 1 , wherein the medical device with the polyelectrolyte LbL coating thereon is prepared by applying alternatively onto a preformed medical device at least one layer of the negatively charged polyionic material having —COOH groups, at least one layer of the positively charged polyionic material, and optionally at least one capping layer of a polyionic material, at least one capping bilayer of two oppositely charged polyionic materials or at least one capping layer of a charged polymeric material and a non-charged polymeric material that can be non-covalently bonded to the charged polymeric material.
3. The method of claim 1 , wherein the medical device with the polyelectrolyte LbL coating thereon is prepared by:
(a) forming a mold for making the medical device, wherein the mold comprises a first mold portion defining a first optical surface and a second mold portion defining a second optical surface, wherein said first mold portion and said second mold portion are configured to receive each other such that a medical device-forming cavity is formed between said first optical surface and said second optical surface;
(b) applying a transferable LbL coating, using a layer-by-layer polyelectrolyte deposition technique, onto at least one of said optical surface, wherein the transferable LbL coating comprises one or more bilayers of a negatively charged polyionic material having —COOH groups and a positively charged polyionic material;
(c) positioning said first mold portion and said second mold portion such that said mold portions receive each other and said optical surfaces define said medical device forming cavity;
(d) dispensing a polymerizable composition into said medical device-forming cavity; and
(e) curing said polymerizable composition within said medical device-forming cavity such that the medical device is formed, whereby said transferable LbL coating detaches from said at least one optical surface of said mold portion and reattaches to said formed medical device such that said medical device becomes coated with the polyelectrolyte LbL coating.
4. The method of claim 2 , wherein the polyelectrolyte LbL coating further includes at least one capping layer of a polyionic material, at least one capping bilayer of two oppositely charged polyionic materials or at least one capping layer of a charged polymeric material and a non-charged polymeric material that can be non-covalently bonded to the charged polymeric material.