Compositions and methods for detecting animal byproduct in feed
Analytes and ligands for detecting animal byproduct in agricultural samples such as animal feed to minimize the transmission of prion pathogens into food products consumed by humans. The analyte is a component of rendered animal byproduct such as meat and bone meal (MBM). An antibody specific for the analyte is produced by immunizing an animal with MBM or an immunogenic component of MBM that is found in rendered animal product.
1. A method for detecting rendered animal byproduct in a sample comprising:
combining the sample suspected of containing rendered animal byproduct with a detectable antibody having binding affinity for an analyte for a time and under conditions effective to cause at least some analyte, if present, to bind which at least some antibody to form a complex;
separating unbound antibody from the complex,
determining existence of the complex,
correlating the existence of the complex to determine presence of the analyte in the sample;
wherein the antibody lacks immunoreactivity with animal muscle tissue;
wherein the analyte is a component of rendered animal bone or connective tissue or is a component of the extracellular matrix of bone or cartilage;
wherein the sample is animal feed or a component thereof; and
wherein the amount of rendered animal byproduct detected by the method is about 0.005% to about 0.01% by weight.
2. The method of claim 1 , wherein:
a detectable label is attached to the antibody,
combining the sample with the antibody further comprises combining the sample and antibody with a second antibody that is bound to at least one location on a solid phase for a time and under conditions effective to cause at least some analyte, if present, to bind with at least some antibody and at least some second antibody such that at least some antibody becomes immobilized in the location, and
determining the existence of the complex comprises determining whether detectable label is present in the location.
3. The method of claim 1 , wherein:
a detectable label is attached to the antibody, combining the sample with the antibody further comprises combining the sample and antibody with an analyte analog that is bound to at least one location on a solid phase, wherein the antibody has a binding affinity for the analyte analog, and
determining the existence of the complex comprises determining the amount of labeled antibody present in the location.
4. The method of claim 1 , wherein:
combining the sample with the antibody further comprises combining the sample and antibody with an analyte analog having a detectable label attached thereto and the antibody has a binding affinity for the analyte analog,
the antibody is bound to at least one location on a solid phase,
the method further comprises separating unbound analyte analog from bound analyte analog after the combining step and before the determining step,
determining die existence of the complex comprises determining the amount of labeled analyte analog present in the location.
5. The method of claim 1 , wherein:
determining existence of the complex further comprises determining the amount of the complex, and
correlating the existence of the complex further comprises correlating the amount of complex to determine the amount of analyte present in the sample.
6. The method of claim 1 , wherein the assay further comprises:
combining the sample with at least one additional antibody having binding affinity for a component of rendered animal byproduct of one or more known taxonornic groups, but having measurably lower binding affinity for rendered animal byproduct from one or more different taxonomic groups, for a time and under conditions effective to cause the second antibody to bind with the analyte, if present, to form a complex,
determining existence of the second complex, and
correlating the existence of the second complex to determine presence of rendered animal byproduct of a known taxonomic group or combination of taxonomic groups.
7. The method of claim 2 , wherein the detectable label comprises at least one of radioactive molecules, enzymes, substrates, cofactors, inhibitors, fluorescent moieties, chemiluminescent moieties, or magnetic particles.