IP Library Granted Patent US 7,119,345
Granted Patent B2
US 7,119,345 · App. 10/735,339 · Granted Oct 10, 2006

Excitation and emission filter

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Quick Facts
Patent No.
US 7,119,345
App. No.
10/735,339
Granted
Oct 10, 2006
Kind
B2
Abstract

Systems and methods for detecting fluorescent light by providing a filter assembly including an excitation filter and an emission filter.

Claims (32)

1. A fluorescent detection system comprising:

an array of excitation light sources;

an array of detectors; and

a filter assembly comprising an excitation filter positioned for excitation light from the excitation light sources and an emission filter positioned for fluorescent light from an array of samples,

wherein the excitation filter and emission filter are positioned substantially parallel to each other, and

wherein the filter assembly comprises alternating portions of excitation filter and portions of emission filter.

2. The fluorescent detection system of claim 1 , wherein each sample corresponds to one excitation light source and two detectors.

3. The fluorescent detection systein of claim 1 , wherein each sample corresponds to two excitation light sources and one detector.

4. The fluorescent detection system of claim 3 , wherein the two excitation light sources are narrow band sources and the one detector is a broad band detector.

5. The fluorescent detection system of claim 4 , wherein the excitation filter substantially rejects wavelengths outside those of the narrow band sources.

6. The fluorescent detection system of claim 5 , wherein the emission filter substantially rejects wavelengths of the narrow band sources.

7. The analytical instrument of claim 6 , wherein the excitation filter has a maximum wavelength of at most 535 nm and the excitation filter exhibits at least 80% efficiency in rejecting wavelengths greater than the maximum wavelength.

8. The analytical instrument of claim 6 , wherein the emission filter has a minimum wavelength of at least 535 nm and the excitation filter exhibits at least about 80% efficiency in rejecting wavelengths less than the minimum wavelength.

9. The analytical instrument of claim 8 , wherein the maximum wavelength is 10 nm or more shorter than the minimum wavelength.

10. The analytical instrument of claim 1 , wherein the alternating portions are positioned in strips.

11. The analytical instrument of claim 1 , wherein the alternating portions are positioned in an staggered array.

12. The fluorescent detection system of claim 1 , further comprising an array of focusing lenses.

13. The fluorescent detection system of claim 12 , wherein each focusing lens corresponds to each sample.

14. The fluorescent detection system of claim 12 , wherein a first set of focusing lenses corresponds to the excitation light and a second set of the focusing lenses corresponds to the fluorescent light.

15. The fluorescent detection system of claim 1 , further comprising an array of collimating lenses.

16. The fluorescent detection system of claim 15 , wherein the array of collimating lenses comprises a first set of collimating lenses for the excitation light and a second set of collimating lenses for the fluorescent light.

17. The filter assembly of claim 1 , wherein the excitation filter and the emission filter are part of one substrate.

18. The filter assembly of claim 17 , wherein the excitation filter comprises a coating on the substrate.

19. The filter assembly of claim 17 , wherein the emission filter comprises a coating on the substrate.

20. The filter assembly of claim 17 , wherein the substrate comprises a first layer configured to provide the excitation filter and a second layer configured to provide the emission filter.

21. A filter assembly comprising:

an excitation filter adapted to condition excitation light from an excitation light source; and

an emission filter adapted to condition fluorescent light from a sample,

wherein the excitation filter and the emission filter are form alternating portions of the filter assembly, wherein the filter assembly is substantially flat, and

wherein the excitation filter and the emission filter form a lattice with nodes and openings.

22. The filter assembly of claim 21 , wherein the excitation filter and emission filter are separate structures coupled together.

23. The filter assembly of claim 21 , wherein the nodes and openings are positioned in a staggered array.

Assignments (5)
CORRECTIVE ASSIGNMENT TO CORRECT THE RECEIVING PARTY NAME PREVIOUSLY RECORDED AT REEL: 030182 FRAME: 0677. ASSIGNOR(S) HEREBY CONFIRMS THE RELEASE OF SECURITY INTEREST. Recorded Mar 4, 2016
From: BANK OF AMERICA, N.A.
To: APPLIED BIOSYSTEMS, LLC
Reel/Frame 038002/0697 →
LIEN RELEASE Recorded Apr 9, 2013
From: BANK OF AMERICA, N.A.
To: APPLIED BIOSYSTEMS, INC.
Reel/Frame 030182/0677 →
CHANGE OF NAME Recorded Feb 26, 2010
From: APPLERA CORPORATION
To: APPLIED BIOSYSTEMS INC.
Reel/Frame 023994/0538 →
MERGER Recorded Feb 26, 2010
From: APPLIED BIOSYSTEMS INC.
To: APPLIED BIOSYSTEMS, LLC
Reel/Frame 023994/0587 →
SECURITY AGREEMENT Recorded Dec 5, 2008
From: APPLIED BIOSYSTEMS, LLC
To: BANK OF AMERICA, N.A, AS COLLATERAL AGENT
Reel/Frame 021976/0001 →