Activated polyethylene glycol compounds
View Patent ↗Novel arylisothiocyanate compounds are described that are useful for activating alcohol-containing macromolecules, for example polyethyleneglycols and cellulose, for covalent linkage to amino-groups of biomolecules, for example polypeptides such as antibodies, enzymes, and proteins.
1. A compound represented by the formula
where B is an amino group-containing biomolecule;
R is —NHC(O)—O—M;
where n=1 or 2;
M is a polyethylene glycol or a polysaccharide; and
—R is para, meta or di-meta relative to —NHC(S)—NH—B.
2. The compound according to claim 1 , where M is methoxy polyethylene glycol.
3. The compound according to claim 1 wherein said biomolecule B is streptavidin.
4. The compound according to claim 1 , wherein the polysaccharide is dextran, cellulose, starch or agarose.
5. The compound according to claim 2 , wherein the methoxy polyethylene glycol is —CH2CH2—(OCH2CH2) x —O—CH3 and x is an average value that is about 5 to about 500.
6. A compound represented by the formula
wherein x is an average value that is about 5 to about 500 and wherein B is an amino group-containing biomolecule.
7. The compound of claim 1 , wherein B is a protein or a polypeptide.
8. The compound of claim 6 , wherein B is a protein or a polypeptide.