Granted Patent B2

US 7,001,430 · App. 10/754,310 · Granted Feb 21, 2006

Abstract

Claims (31)

1. An intermediate composition for producing a matrix suitable implantation in humans, comprising shredded, allogeneic human muscle tissue that has been combined with an aqueous carrier to form a muscle tissue slurry having a viscosity within the range of 1 centistoke to 20,000 centistokes at 25° C., wherein said shredded allogeneic human muscle tissue is lyophilized and shredded into a coarse fiber prior to said digestion with a biocompatible acid.

2. The composition of claim 1 wherein said muscle tissue slurry having a viscosity within the range of 1 centistoke to 10,000 centistokes at 25° C.

3. The composition of claim 2 , wherein said muscle tissue slurry having a viscosity within the range of 1 centistoke to 5,000 centistokes at 25° C.

4. The composition of claim 1 wherein said shredded allogeneic human muscle tissue is also defatted.

5. The composition of claim 1 wherein said aqueous carrier comprises a biocompatible acid selected from the group consisting of acetic acid, citric acid, formic acid, hydrochloric acid, phosphoric acid, phosphorus acid and sulfuric acid.

6. The composition of claim 1 wherein the biocompatible acid is an organic acid.

7. The composition of claim 6 , wherein the organic acid is acetic acid.

8. A tissue implant suitable for treating an injury or a medical condition in a human comprising a matrix of digested allogeneic human muscle.

9. The implant of claim 8 , wherein said matrix of digested allogeneic human muscle comprises from about 1% to about 100% of the final weight of said implant.

10. The implant of claim 9 , wherein said matrix of digested allogeneic human muscle comprises from 15% to about 95% of the weight of said tissue implant.

11. The implant of claim 10 , wherein said matrix of digested allogeneic human muscle comprises from 25% to about 85% of the weight of said tissue implant.

12. The implant of claim 8 , further comprising demineralized bone matrix (DBM).

13. The implant of claim 12 , wherein said DBM is uniformly distributed throughout the matrix.

14. The implant of claim 12 , wherein said DBM is layered within said matrix.

15. The implant of claim 8 , further comprising cortical cancellous chips (CCC).

16. The implant of claim 15 , wherein said CCC is uniformly distributed throughout the matrix.

17. The implant of claim 15 , wherein said CCC is layered within said matrix.

18. The implant of claim 8 further comprising digested allogeneic human tendon.

19. A method for making a muscle tissue slurry comprising the steps of:

i. removing the fat and soluble proteins from allogeneic or xenographic mammalian muscle tissue;

ii. lyophilizing the muscle tissue from step (i);

iii. shredding the lyophilized muscle tissue; and

iv. mixing the shredded muscle tissue in an aqueous carrier to form a muscle tissue slurry having a viscosity within the range of 1 centistoke to 20,000 centistoke.

20. A method for making an implantable tissue comprising the steps of:

i. removing the fat and soluble proteins from allogeneic or xenographic mammalian muscle tissue;

ii. lyophilizing the muscle tissue from step (i);

iii. shredding the lyophilized muscle tissue;

iv. mixing the shredded muscle tissue in an aqueous carrier to form a muscle tissue slurry having a viscosity within the range of 1 centistoke to 20,000 centistokes;

v. transferring the muscle tissue slurry to an appropriate shaped mold; and

vi. drying the slurry in the mold to form the correspondingly shaped tissue implant.

21. The method of claim 20 , wherein the aqueous carrier comprises a biocompatible acid.

Assignments (23)