Post-transcriptional gene silencing using expressed double stranded RNA
View Patent ↗Described herein are methods for identifying nucleic acid sequences that modulate the function of a cell, the expression of a gene in a cell, or the biological activity of a target polypeptide in a cell. The methods involve the use of double stranded RNA expression libraries, double stranded RNA molecules, and post-transcriptional gene silencing techniques.
1. A method comprising:
introducing into a population of RNA stress response-competent cells an expression vector comprising a promoter sequence operably linked to a coding sequence for a nucleic acid less than 50 nucleotides in length for expression of a double stranded hairpin RNA, wherein one strand of the double stranded portion of the hairpin RNA is complementary to a target gene mRNA in the cell, and wherein the hairpin RNA downregulates expression of the target RNA when expressed from the introduced expression vector, wherein the level of apoptosis in the population of cells in which the hairpin RNA is expressed is lower than the level of apoptosis in a population of cells transfected with the same double stranded hairpin RNA produced outside the cell.
2. The method of claim 1 , wherein said target gene is a pathogen gene.
3. The method of claim 1 , wherein said RNA stress response-competent cell is a vertebrate cell.
4. The method of claim 3 , wherein said vertebrate RNA stress response-competent cell is a mammalian cell.
5. The method of claim 1 , wherein said double stranded hairpin RNA is expressed as a single transcript that contains an inverted repeat.
6. The method of claim 1 , wherein expression of said target gene is decreased at least 50%.
7. The method of claim 6 , wherein expression of said target gene is decreased 90%.
8. The method of claim 1 , wherein the population of RNA stress response-competent cells is ex vivo.