Highly-packed polycationic ammonium, sulfonium and phosphonium lipids
View Patent ↗The present invention discloses highly packed polycationic ammonium, sulfonium and phosphonium lipid compounds useful for making lipid aggregates for delivery of macromolecules and other compounds into cells. They are especially useful for the DNA-dependent transformation of cells. Methods for their preparation and use as intracellular delivery agents are also disclosed.
1. A compound or polycation having the formula:
or salt thereof where:
x is an integer ranging from 1 to about 20;
l is an integer ranging from 1 to about 6;
r and s, independently of one another, are 0 or 1, wherein when r is 1, the N bonded to R 1 and A 1 has a positive charge, and when s is 1, the N bonded to R 2 and A 2 has a positive charge;
R A and R B , independently of one another, are selected from the group consisting of H, or an alkyl, hydroalkyl or thiol-substituted alkyl group having from 1 to 6 carbon atoms;
R 1 and R 2 , independently of one another, are selected from the group consisting of alkyl groups having 1 to about 6 carbon atoms; and
A 1 and A 2 , independently of other A 1 and A 2 groups, are selected from the group consisting of a —CH(D-L) 2 and a —C(D-L) 3 group wherein D is selected from the group consisting of —CO—, —CO 2 —, —O—C—O—, —CO—N—, —O—CO—N—, —O—, and —S—, and L is selected from the group consisting of:
(a) a straight chain or branched alkyl, alkenyl, or alkynyl group having from 2 to about 22 carbon atoms wherein one or more non-neighboring —CH 2 — groups can be replaced with an O or S atom;
(b) a substituted straight chain or branched alkyl, alkenyl, or alkynyl group having from 2 to about 22 carbon atoms wherein the substituent is an aromatic, alicyclic heterocyclic or polycyclic ring and wherein one or more of the non-neighboring neighboring —CH 2 — groups of said alkyl, alkenyl or alkynyl group can be substituted with an O or S atom; and
(c) an aromatic, alicyclic, heterocyclic and a polycyclic ring moiety.
2. The compound or polycation of claim 1 wherein L is selected from the group consisting of a straight chain or branched alkyl, alkenyl, or alkynyl group having from 2 to about 22 carbon atoms wherein one or more non-neighboring —CH 2 — groups can be replaced with an O or S atom.
3. The compound or polycation of claim 1 wherein L is selected from the group consisting of a substituted straight chain or branched alkyl, alkenyl, or alkynyl group having from 2 to about 22 carbon atoms wherein the substituent is an aromatic, alicyclic heterocyclic or polycyclic ring and wherein one or more of the non-neighboring —CH 2 — groups of said alkyl, alkenyl or alkynyl group can be substituted with an O or S atom.
4. A lipid aggregate which comprises one or more compounds of claim 1 .