IP Library › Granted Patent US 6,955,906
Granted Patent B2
US 6,955,906 · App. 10/763,933 · Granted Oct 18, 2005

Biotin biosynthetic genes having biotin synthase activity

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Quick Facts
Patent No.
US 6,955,906
App. No.
10/763,933
Granted
Oct 18, 2005
Kind
B2
Abstract

The present invention relates to the production process of biotin by fermentation using a genetically engineered microorganism, and DNA sequences and vectors to be used in such process.

Claims (20)

1. An isolated DNA molecule comprising a polynucleotide selected from the group consisting of:

a) SEQ ID NO: 7;

b) a polynucleotide which encodes the polypeptide of SEQ ID NO: 8; and

c) a polynucleotide which hybridizes to the complement of the polynucleotide from a) or b) under stringent hybridizing conditions, wherein the stringent conditions include hybridizing and washing in 0.2×SSC at about 65° C. and wherein the polynucleotide encodes a polypeptide having biotin synthase activity.

2. The isolated DNA molecule of claim 1 which comprises SEQ ID NO: 7.

3. An expression vector comprising a polynucleotide selected from the group consisting of:

a) SEQ ID NO: 7;

b) a polynucleotide which encodes the polypeptide of SEQ ID NO: 8; and

c) a polynucleotide which hybridizes to the complement of the polynucleotide from a) or b) under stringent hybridizing conditions, wherein the stringent conditions include hybridizing and washing in 0.2×SSC at about 65° C. and wherein the polynucleotide encodes a polypeptide having biotin synthase activity.

4. The expression vector of claim 3 which comprises SEQ ID NO: 7.

5. A biotin-expressing cell transformed with an expression vector comprising polynucleotide selected from the group consisting of:

a) SEQ ID NO: 7;

b) a polynucleotide which encodes the polypeptide of SEQ ID NO: 8; and

c) a polynucleotide which hybridizes to the complement of the polynucleotide from a) or b) under stringent hybridizing conditions, wherein the stringent conditions include hybridizing and washing in 0.2×SSC at about 65° C. and wherein the polynucleotide encodes a polypeptide having biotin synthase activity.

6. The cell of claim 5 in which the expression vector comprises SEQ ID NO: 7.

7. A process for the production of biotin comprising culturing a biotin-expressing cell transformed by an expression vector, wherein the expression vector comprises a polynucleotide selected from the group consisting of:

a) SEQ ID NO: 7;

b) a polynucleotide which encodes the polypeptide of SEQ ID NO: 8; and

c) a polynucleotide which hybridizes to the complement of the polynucleotide from a) or b) under stringent hybridizing conditions, wherein the stringent conditions include hybridizing and washing in 0.2×SSC at about 65° C. and wherein the polynucleotide encodes a polypeptide having biotin synthase activity.

8. The process of claim 7 in which the expression vector comprises SEQ ID NO: 7.