Membrane translocating peptide drug delivery system
View Patent ↗The present invention relates to a novel membrane translocating full-length peptide sequence, fragment, motif, derivative, analog or peptidomimetic thereof (MTLPs), to nucleotide sequences coding therefor, and to compositions comprising a MTLP-active agent complex and a MTLP-active particle complex. The MTLP or the nucleotide sequence coding therefor enhance movement of the active agent or of the active particle across a lipid membrane. More particularly, the present invention relates to a MTLP-active agent complex and a MTLP-active particle complex, wherein the MTLP enhances uptake of the active agent into a cell, into or out of an intracellular compartment and across a cell layer. Methods of making and methods of using MTLPs also are included.
1. A composition comprising a peptide having the amino acid sequence as set forth in SEQ ID NO: 24 and a carrier.
2. The composition of claim 1 , further comprising an active agent, selected from the group consisting of therapeutic agent, prophylactic agent, and diagnostic agent, wherein said peptide is complexed to said active agent.
3. A composition according to claim 2 , wherein said active agent comprising DNA is complexed with a membrane translocating peptide (MTLP)-coated liposome.
4. The composition of claim 1 , further comprising an active particle, selected from the group consisting of nanoparticle, liposome, and microparticle, wherein said peptide is complexed to said active particle.
5. A composition for use in membrane translocation, the composition consisting of the MTLP comprising an amino acid sequence of SEQ ID NO: 24 and a carrier.
6. A composition of claim 5 , wherein said MTLP is complexed to a liposome.
7. A pharmaceutical composition comprising the composition according to claim 1 and a pharmaceutical carrier.
8. A pharmaceutical composition according to claim 7 , adapted for oral administration.
9. A pharmaceutical composition comprising a composition according to claim 5 and a pharmaceutical carrier.
10. A pharmaceutical composition according to claim 6 , adapted for oral administration.
11. A method for enhancing movement of an active agent selected from the group consisting of therapeutic agent, prophylactic agent, and diagnostic agent, across a lipid membrane, comprising a administering the composition of claim 2 to said membrane wherein the peptide enhances movement of the active agent across the lipid membrane.
12. A method for enhancing movement of an active particle selected from the group consisting of nanoparticle, liposome, and microparticle, across a lipid membrane, comprising a administering the composition of claim 4 to said membrane wherein the peptide enhances movement of the active particle across the lipid membrane.
13. A method for treating a pathological disorder in an animal, comprising orally administering to the animal in need of such treatment the composition of claim 8 wherein an amount of the active agent effective to treat the pathological disorder is moved across the gastrointestinal epithelium of the animal into the circulation.