Membrane active polymers
View Patent ↗We describe a class of polymaleic anhydride polymers capable of disrupting cell membranes. Co-delivery of these polymers with biologically active compounds increases cellular cytoplasmic delivery of the compounds.
1. A process for delivering a polynucleotide to the cytoplasm of a cell in vitro consisting of:
a) forming a styrene-maleic anhydride random copolymer having 50% styrene and 50% maleic anhydride;
b) increasing hydrophobicity of the copolymer by reacting the anhydride monomers in the copolymer with hydrophobic amines or hydrophobic alcohols thereby forming a membrane active polyanion capable of lysing mammalian cell membranes at pH 6.5; and
c) contacting said cell with said polynucleotide and said membrane active polyanion such that the polynucleotide and the membrane active polyanion are endocytosed by the cell.
2. The process of claim 1 wherein increasing hydrophobicity of the copolymer consists of reacting the anhydride monomers in the copolymer with hydrophobic amines.
3. A process for delivering a polynucleotide to the cytoplasm of a cell in vitro consisting of:
a) forming a butyl vinyl ether-maleic anhydride alternating copolymer;
b) increasing hydrophobicity of the copolymer by reacting the anhydride monomers in the copolymer with hydrophobic amines or hydrophobic alcohols thereby forming a membrane active polyanion capable of lysing mammalian cell membranes at pH 6.5; and
c) contacting said cell with said polynucleotide and said membrane active polyanion such that the polynucleotide and the membrane active polyanion are endocytosed by the cell.
4. The process of claim 3 wherein increasing hydrophobicity of the copolymer consists of reacting the anhydride monomers in the copolymer with hydrophobic amines.