Chemical substance having morphogenetic and growth-accelerating activities
View Patent ↗The present invention relates to a method of producing a novel chemical substance capable of inducing morphogenesis, promoting growth of marine foliate green alga that use the microorganisms, and a culturing media for alga containing these novel chemical substances.
1. An isolated morphogenic and growth accelerating chemical substance 1 having the following physicochemical properties:
(i) color of substance: colorless;
(ii) molecular weight: 457;
(iii) mass spectrometry: FABMS:m/z 456 [M−H] − ( FIG. 1 ); and
(iv) nuclear magnetic resonance signal:
1) 1 H-NMR(D 2 O-20 mM Na 2 HPO 4 (pH 9), 750 MHz): ( FIG. 2 )
δ ppm 0.818 (3H, s), 0.837 (3H, s), 0.882 (3H, s), 0.960 (1H, m), 1.058 (1H, m), 1.167 (1H, m), 1.326 (3H, s), 1.37 (1H, m), 1.38 (1H, m), 1.40 (1H, m), 1.58 (1H, m), 1.61 (2H, m), 1.52 (1H, br d, J=13 Hz), 1.76 (1H, br d, J=14 Hz), 2.024 (1H, m), 2.181 (1H, dd, J=4, 14 Hz), 2.291 (1H, dd, J=14, 16.5 Hz), 7.698 (1H, d, J=7.5 Hz), 7.845(1H, d, J=7.5 Hz); and
2) 13 C-NMR(D 2 O-20 mM Na 2 HPO 4 (pH 9), 125 MHz): ( FIG. 3 )
δ ppm 15.236 (q), 19.037 (t), 20.257 (t), 20.955 (q), 21.835 (q), 25.987 (t), 33.381 (s), 33.636 (q), 37.308 (s), 39.590 (t), 41.199 (t), 42.346 (t), 52.769 (d), 56.381 (d), 79.096 (s), 114.965 (s), 124.399 (d), 139.004 (s), 141.232 (d), 150.282 (s), 152.656 (s), 172.081 (s), 173.538 (s), 174.661 (s).
2. The chemical substance 1 according to claim 1 , which is obtainable from the YM-2-23 strain (FERM BP-8417).
3. The chemical substance 1 according to claim 1 , which is represented by the following formula:
4. The chemical substance 1 according to claim 1 , which has the molecular formula: C 25 H 31 NO 7 .
5. A process for producing a chemical substance 1, wherein microorganisms capable of producing the chemical substance 1 according to claim 1 are cultured in medium, the chemical substance 1 is generated and accumulated in the cultures, and the generated and accumulated novel chemical substance 1 is recovered.
6. The production process according to claim 5 , wherein the microorganisms are YM2-23 strain (FERM BP-8417), or an analogous strain thereof.
7. Culture medium for algae comprising, as an active ingredient, the chemical substance 1 according to claim 1 .
8. A monomethylated, dimethylated, or trimethylated form of the chemical substance 1 obtained by treating the chemical substance 1 according to claim 1 with trimethylsilyldiazomethane.
9. A compound or a derivative thereof obtained by treating the trimethylated form according to claim 8 with sodium borohydride.