Apparatus for measuring fluorescence lifetime
View Patent ↗An apparatus for measuring fluorescence lifetime includes a pulse laser source that generates a pulse excitation light, a detector that detects a fluorescence generated from a sample by irradiating the pulse excitation light, and outputs a detection signal corresponding to the fluorescence, a measurement unit that measures number of photons emitted in a predetermined time gate based on the detection signal, a correction unit that corrects the number of photons measured based on Poisson distribution, and a calculation unit that calculates the fluorescence lifetime of the sample based on the number of photons corrected.
1. An apparatus for measuring fluorescence lifetime, comprising:
a pulse laser source that generates a pulse excitation light;
an optical system that irradiates a sample with the pulse excitation light;
a detector which detects a fluorescence emitted from the sample, and which outputs a detection signal corresponding to the fluorescence;
a measurement unit which detects, based on the detection signal, whether the fluorescence is emitted for each of a plurality of time gates starting at different times, and which measures a number of photons of the fluorescence for each time gate;
a correction unit which corrects, based on a predetermined probability distribution for a probability of emitting photons, at least one of: (i) the number of photons measured by the measurement unit for each time gate, and (ii) a first ratio of the number of photons measured by the measurement unit to a predetermined number of irradiations of the pulse excitation light into a second ratio, for each time gate; and
a calculation unit that calculates a fluorescence lifetime of the sample based on at least one of the corrected number of photons and the second ratio.
2. The apparatus according to claim 1 , wherein the predetermined probability distribution is Poisson distribution.
3. A method of measuring fluorescence lifetime, comprising:
irradiating a sample with a pulse excitation light;
detecting a fluorescence emitted from the sample, and outputting a detection signal corresponding to the fluorescence;
detecting, based on the detection signal, whether the fluorescence is emitted for each of a plurality of time gates starting at different times;
measuring a number of photons of the fluorescence for each time gate;
correcting, based on a predetermined probability distribution for a probability of emitting photons, at least one of: (i) the measured number of photons for each time gate, and (ii) a first ratio of the measured number of photons to a predetermined number of irradiations of the pulse excitation light into a second ratio, for each time gate; and
calculating a fluorescence lifetime based on at least one of the corrected number of photons and the second ratio.
4. The method according to claim 3 , wherein the predetermined probability distribution is Poisson distribution.