Polymorphs of an epothilone analog
View Patent ↗There are provided in accordance with the present invention two crystalline polymorphs, designated Form A and Form B, respectively, as well as mixtures thereof, of an epothilone analog represented by the formula Also provided are methods of forming the novel polymorphs, therapeutic methods utilizing them and pharmaceutical dosage forms containing them.
1. A method for treating cancer or other proliferative diseases in a mammal, comprising:
a) preparing a pharmaceutical composition comprising an active ingredient and one or more pharmaceutically acceptable carriers, excipients or diluents thereof; wherein the active ingredient comprises an effective amount of a crystalline material of an epothilone analog represented by formula I:
wherein the crystalline material is Form A and optionally Form B; and
b) administrating the pharmaceutical composition to the mammal;
wherein the Form A is characterized by:
i) unit cell parameters approximately equal to the following:
Cell dimensions
a = 14.152(6) Å
b = 30.72(2) Å
c = 6.212(3) Å
Volume = 2701(4) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.247
Melting point
182-185° C. (decomposition);
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 5.69, 6.76, 8.38, 11.43, 12.74, 13.62, 14.35, 15.09, 15.66, 16.43, 17.16, 17.66, 18.31, 19.03, 19.54, 20.57, 21.06, 21.29, 22.31, 23.02, 23.66, 24.18, 14.98, 25.50, 26.23, 26.23, 26.46, 27.59, 28.89, 29.58, 30.32, 31.08 and 31.52; and/or
ii) a powder x-ray diffraction substantially as shown in FIG. 1 and a Raman spectrum substantially as shown in FIG. 5 ; and/or
iii) a solubility in water of 0.1254, a solubility in a 3% aqueous solution of polysorbate 80 of 0.2511, a melting point with decomposition between 182-185° C. and a heat of solution of 20.6 kJ/mol; and
wherein the Form B, if present, is characterized by:
i) unit cell parameters approximately equal to the following:
Cell dimensions
a = 16.675(2) Å
b = 28.083(4) Å
c = 6.054(1) Å
Volume = 2835(1) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.187
Melting point
191-199° C. decomposition;
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 6.17, 10.72, 12.33, 14.17, 14.93, 15.88, 16.17, 17.11, 17.98, 19.01, 19.61, 20.38, 21.55, 21.73, 22.48, 23.34, 23.93, 24.78, 25.15, 25.90, 26.63, 27.59, 28.66, 29.55, 30.49 and 31.22; and/or
ii) a powder x-ray diffraction substantially as shown in FIG. 2 and a Raman spectrum substantially as shown in FIG. 6 ; and/or
iii) a solubility in water of 0.1907, a solubility in a 3% aqueous solution of polysorbate 80 of 0.5799, a melting point with decomposition between 191-199° C. and a heat of solution of 9.86 kJ/mol.
2. The method according to claim 1 wherein the Form A is characterized by:
unit cell parameters approximately equal to the following:
Cell dimensions
a = 14.152(6) Å
b = 30.72(2) Å
c = 6.212(3) Å
Volume = 2701(4) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.247
Melting point
182-185° C. (decomposition);
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 5.69, 6.76, 8.38, 11.43, 12.74, 13.62, 14.35, 15.09, 15.66, 16.43, 17.16, 17.66, 18.31, 19.03, 19.54, 20.57, 21.06, 21.29, 22.31, 23.02, 23.66, 24.18, 14.98, 25.50, 26.23, 26.23, 26.46, 27.59, 28.89, 29.58, 30.32, 31.08 and 31.52.
3. The method according to claim 1 wherein the Form A is characterized by: a powder x-ray diffraction substantially as shown in FIG. 1 and a Raman spectrum substantially as shown in FIG. 5 .
4. The method according to claim 1 wherein the Form A is characterized by: a solubility in water of 0.1254, a solubility in a 3% aqueous solution of polysorbate 80 of 0.2511, a melting point with decomposition between 182-185° C. and a heat of solution of 20.6 kJ/mol.
5. The method according to claim 1 wherein the mammal is a human.
6. The method according to claim 5 wherein the effective amount is in the range of from about 0.05 to about 200 mg/kg/day.
7. The method according to claim 1 wherein the cancer is breast cancer or lung cancer.
8. The method according to claim 1 wherein the pharmaceutical composition is administered parenterally.
9. The method according to claim 1 wherein the pharmaceutical composition comprises the Form A and the Form B.
10. The method according to claim 9 wherein the mammal is a human and the effective amount is in the range of from about 0.05 to about 200 mg/kg/day.
11. The method according to claim 9 wherein the cancer is breast cancer or lung cancer.
12. A method for treating cancer or other proliferative diseases in a mammal, comprising:
a) preparing a pharmaceutical composition comprising an active ingredient and one or more pharmaceutically acceptable carriers, excipients or diluents thereof; wherein the active ingredient comprises an effective amount of a crystalline material of an epothilone analog represented by formula I:
wherein the crystalline material is Form B and optionally Form A; and
b) administrating the pharmaceutical composition to the mammal;
wherein the Form A, if present, is characterized by:
i) unit cell parameters approximately equal to the following:
Cell dimensions
a = 14.152(6) Å
b = 30.72(2) Å
c = 6.212(3) Å
Volume = 2701(4) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.247
Melting point
182-185° C. (decomposition);
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 5.69, 6.76, 8.38, 11.43, 12.74, 13.62, 14.35, 15.09, 15.66, 16.43, 17.16, 17.66, 18.31, 19.03, 19.54, 20.57, 21.06, 21.29, 22.31, 23.02, 23.66, 24.18, 14.98, 25.50, 26.23, 26.23, 26.46, 27.59, 28.89, 29.58, 30.32, 31.08 and 31.52; and/or
ii) a powder x-ray diffraction substantially as shown in FIG. 1 and a Raman spectrum substantially as shown in FIG. 5 ; and/or
iii) a solubility in water of 0.1254, a solubility in a 3% aqueous solution of polysorbate 80 of 0.2511, a melting point with decomposition between 182-185° C. and a heat of solution of 20.6 kJ/mol; and
wherein the Form B is characterized by:
i) unit cell parameters approximately equal to the following:
Cell dimensions
a = 16.675(2) Å
b = 28.083(4) Å
c = 6.054(1) Å
Volume = 2835(1) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.187
Melting point
191-199° C. decomposition;
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 6.17, 10.72, 12.33, 14.17, 14.93, 15.88, 16.17, 17.11, 17.98, 19.01, 19.61, 20.38, 21.55, 21.73, 22.48, 23.34, 23.93, 24.78, 25.15, 25.90, 26.63, 27.59, 28.66, 29.55, 30.49 and 31.22; and/or
ii) a powder x-ray diffraction substantially as shown in FIG. 2 and a Raman spectrum substantially as shown in FIG. 6 ; and/or
iii) a solubility in water of 0.1907, a solubility in a 3% aqueous solution of polysorbate 80 of 0.5799, a melting point with decomposition between 191-199° C. and a heat of solution of 9.86 kJ/mol.
13. A process for preparing a pharmaceutical composition comprising: mixing an active ingredient with one or more pharmaceutically acceptable carriers, excipients or diluents thereof;
wherein the active ingredient comprises an effective amount of a crystalline material of an epothilone analog represented by formula I:
wherein the crystalline material is Form A and optionally Form B:
wherein the Form A is characterized by:
i) unit cell parameters approximately equal to the following:
Cell dimensions
a = 14.152(6) Å
b = 30.72(2) Å
c = 6.212(3) Å
Volume = 2701(4) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.247
Melting point
182-185° C. (decomposition);
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 5.69, 6.76, 8.38, 11.43, 12.74, 13.62, 14.35, 15.09, 15.66, 16.43, 17.16, 17.66, 18.31, 19.03, 19.54, 20.57, 21.06, 21.29, 22.31, 23.02, 23.66, 24.18, 14.98, 25.50, 26.23, 26.23, 26.46, 27.59, 28.89, 29.58, 30.32, 31.08 and 31.52;
ii) a powder x-ray diffraction substantially as shown in FIG. 1 and a Raman spectrum substantially as shown in FIG. 5 ; or
iii) a solubility in water of 0.1254, a solubility in a 3% aqueous solution of polysorbate 80 of 0.2511, a melting point with decomposition between 182-185° C. and a heat of solution of 20.6 kJ/mol; and
wherein Form B, if present, is characterized by:
i) unit cell parameters approximately equal to the following:
Cell dimensions
a = 16.675(2) Å
b = 28.083(4) Å
c = 6.054(1) Å
Volume = 2835(1) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.187
Melting point
191-199° C. decomposition;
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 6.17, 10.72, 12.33, 14.17, 14.93, 15.88, 16.17, 17.11, 17.98, 19.01, 19.61, 20.38, 21.55, 21.73, 22.48, 23.34, 23.93, 24.78, 25.15, 25.90, 26.63, 27.59, 28.66, 29.55, 30.49 and 31.22;
ii) a powder x-ray diffraction substantially as shown in FIG. 2 and a Raman spectrum substantially as shown in FIG. 6 ; or
iii) a solubility in water of 0.1907, a solubility in a 3% aqueous solution of polysorbate 80 of 0.5799, a melting point with decomposition between 191-199° C. and a heat of solution of 9.86 kJ/mol.
14. The process according to claim 13 wherein the Form A is characterized by: unit cell parameters approximately equal to the following:
Cell dimensions
a = 14.152(6) Å
b = 30.72(2) Å
c = 6.212(3) Å
Volume = 2701(4) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.247
Melting point
182-185° C. (decomposition);
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 5.69, 6.76, 8.38, 11.43, 12.74, 13.62, 14.35, 15.09, 15.66, 16.43, 17.16, 17.66, 18.31, 19.03, 19.54, 20.57, 21.06, 21.29, 22.31, 23.02, 23.66, 24.18, 14.98, 25.50, 26.23, 26.23, 26.46, 27.59, 28.89, 29.58, 30.32, 31.08 and 31.52.
15. The process according to claim 13 wherein the Form A is characterized by: a powder x-ray diffraction substantially as shown in FIG. 1 and a Raman spectrum substantially as shown in FIG. 5 .
16. The process according to claim 13 wherein the Form A is characterized by: a solubility in water of 0.1254, a solubility in a 3% aqueous solution of polysorbate 80 of 0.2511, a melting point with decomposition between 182-185° C. and a heat of solution of 20.6 kJ/mol.
17. The process according to claim 13 wherein the pharmaceutical composition comprises the Form A and the Form B.
18. The process according to claim 17 wherein the Form B is characterized by: unit cell parameters approximately equal to the following:
Cell dimensions
a = 16.675(2) Å
b = 28.083(4) Å
c = 6.054(1) Å
Volume = 2835(1) A 3
Space group
P2 1 2 1 2 1
Orthorhombic
Molecules/unit cell
4
Density (calculated) (g/cm 3 )
1.187
Melting point
191-199° C. decomposition;
and characteristic peaks in the powder x-ray diffraction pattern at values of two theta (CuKα λ=1.5406 Å at 22° C.): 6.17, 10.72, 12.33, 14.17, 14.93, 15.88, 16.17, 17.11, 17.98, 19.01, 19.61, 20.38, 21.55, 21.73, 22.48, 23.34, 23.93, 24.78, 25.15, 25.90, 26.63, 27.59, 28.66, 29.55, 30.49 and 31.22.
19. The process according to claim 17 wherein the Form B is characterized by: a powder x-ray diffraction substantially as shown in FIG. 2 and a Raman spectrum substantially as shown in FIG. 6 .
20. The process according to claim 17 wherein the Form B is characterized by: a solubility in water of 0.1907, a solubility in a 3% aqueous solution of polysorbate 80 of 0.5799, a melting point with decomposition between 191-199° C. and a heat of solution of 9.86 kJ/mol.