Platelet concentration syringe kit
View Patent ↗A single-use system for separating blood and producing platelet concentrates includes an elongated container for receiving blood from a patient. The container has a movable plunger mounted within the blood container for expressing blood fractions separated during centrifugation of the container through a first port mounted at one end of said container, a second port mounted on the plunger, and a third port mounted on a plunger rod attached to the plunger.
1. A method of collecting and separating a patient's blood and recovering a platelet-rich concentrate comprising:
(a) collecting the patient's blood using a needle set comprising a hollow needle having attached tubing and a fitting adapted to engage a first port in an elongated container fitted with a movable plunger having a second port therein;
(b) transferring said blood through said first port into said elongated container;
(c) centrifuging said blood in said container and separating said blood into platelet-rich plasma and red blood cells;
(d) displacing the red blood cells separated in (c) from said container by moving said plunger towards said first port and expelling said red blood cells into a waste bag through tubing attached to said first port;
(e) removing said waste bag of (d) and centrifuging said platelet-rich plasma remaining in said container and separating a platelet-rich concentrate from a platelet-poor plasma;
(f) attaching a hollow plunger rod having a third port therein to said plunger and displacing the platelet-poor plasma separated in (e) from said container by moving said plunger towards said first port and expelling said platelet-poor plasma through said second port of said plunger and said third port of said plunger rod into a waste bag attached to the plunger rod;
(g) recovering the platelet-rich concentrate separated in (e) and remaining in said container; and
wherein displacing said red blood cells, platelet-rich plasma, and platelet-poor plasma is carried out automatically in centrifuge equipment having facilities for opening said ports.
2. The method of claim 1 wherein said elongated container contains a small amount of a blood anti-coagulant.
3. The method of claim 1 , wherein the first port includes a valve, the method further comprising opening the valve prior to the act of transferring said blood.
4. The method of claim 1 , wherein said centrifuging of step (c) is a soft spin.
5. A method of collecting and separating a patient's blood and recovering a platelet-rich concentrate comprising:
(a) collecting the patient's blood using a needle set comprising a hollow needle having attached tubing and a fitting adapted to engage a first port in an elongated container fitted with a movable plunger having a second port therein;
(b) opening a valve positioned within the first port;
(c) transferring said blood through said first port into said elongated container by moving the plunger away from the first port;
(d) closing the valve;
(e) centrifuging said blood in said container and separating said blood into platelet-poor plasma, red blood cells, and platelet-rich concentrate;
(f) displacing the red blood cells separated in (e) from said container by moving said plunger towards said first port and expelling said red blood cells into a waste bag through tubing attached to said first port;
(g) removing said waste bag of (f) and attaching a hollow plunger rod having a third port therein to said plunger and displacing the platelet-poor plasma separated in (c) from said container by moving said plunger toward said first port and expelling said platelet-poor plasma through said second port of said plunger and said third port of said plunger rod into a waste bag attached to the plunger rod; and
(h) recovering the platelet-rich concentrate separated in (e) and remaining in said container,
wherein displacing said red blood cells, platelet-rich plasma, and platelet-poor plasma is carried out automatically in centrifuge equipment having facilities for opening said ports.
6. The method of claim 5 wherein said elongated container contains a small amount of a blood anti-coagulant.
7. The method of claim 5 , wherein said centrifuging of step (e) is a heavy spin.