Signatures of ER status in breast cancer
The invention relates to the identification and use of gene expression profiles, or patterns, suitable for identification of populations that are positive and negative for estrogen receptor expression. The gene expression profiles may be embodied in nucleic acid expression, protein expression, or other expression formats, and may be used in the study and/or diagnosis of cells and tissue in breast cancer as well as for the study and/or determination of prognosis of a patient, including breast cancer survival.
1 . An array comprising polynucleotide probes capable of hybridizing to nucleic acid molecules of one or more of the genes listed in Table 1, 2, 3, or 4 and comprising sequences within 350 nucleotides of the polyadenylation site of said genes, said probes hybridized to nucleic acids derived from a cell of a subject afflicted with, or suspected of having, breast cancer
2 . The array of claim 1 comprising 11 or more of the genes in Table 1, 2, 3, or 4.
3 . The array of claim 2 comprising all the genes in Table 1, 2, 3, or 4.
4 . The array of claim 1 wherein said cell is from a human subject or such a subject afflicted with breast cancer.
5 . The array of claim 1 wherein said nucleic acids derived from one or more breast cancer cells are prepared by mRNA amplification.
6 . The array of claim 1 wherein said nucleic acids derived from one or more breast cancer cells are cDNA.
7 . The array of claim 1 wherein said cell is in a section of tissue from a subject or is microdissected from said section.
8 . A method to determine the ER status of breast cancer cells in a sample from a subject comprising assaying said sample for expression of one or more genes in Table 1 or 3 and/or one or more genes in Table 2 or 4.
9 . The method of claim 8 wherein said assaying comprises preparing RNA, optionally labeled, from said sample and optionally converting said RNA into cDNA, optionally labeled.
10 . The method of claim 9 wherein said RNA is not labeled and used for quantitative PCR.
11 . The method of claim 9 wherein said assaying comprises using an array.
12 . The method of claim 8 wherein said sample is a ductal lavage or fine needle aspiration or FFPE breast tissue sample.
13 . The method of claim 12 wherein said sample is microdissected to isolate one or more cells that are breast cancer cells or suspected of being breast cancer cells.
14 . The method of claim 10 further comprising determination of the ratio of the expression of a gene in Table 1 or 3 to the expression of a gene in Table 2 or 4 as an indicator of ER status.
15 . A method to determine therapeutic treatment for a patient having breast cancer comprising
identifying said patient as being ER positive or ER negative after assaying breast cancer cells of said patient for expression of one or more genes listed in Table 1, 2, 3, and/or 4 and
selecting the appropriate treatment for a patient having cells of such ER status.
16 . The method of claim 15 wherein said assaying comprises preparing RNA, optionally labeled, from said sample and optionally converting said RNA into cDNA, optionally labeled.
17 . The method of claim 16 wherein said RNA is not labeled and used for quantitative PCR.
18 . The method of claim 16 wherein said assaying comprises using an array.
19 . The method of claim 16 wherein said sample is a ductal lavage or fine needle aspiration or FFPE breast tissue sample.
20 . The method of claim 19 wherein said sample is microdissected to isolate one or more cells that are breast cancer cells or suspected of being breast cancer cells.
21 . The method of claim 16 wherein said assaying comprises determination of the ratio of the expression of a gene in Table 1 or 3 to the expression of a gene in Table 2 or 4 as an indicator of ER status.