Isolated human drug-metabolizing proteins, nucleic acid molecules encoding human drug-metabolizing proteins, and uses thereof
View Patent ↗The present invention provides amino acid sequences of peptides that are encoded by genes within the human genome, the drug-metabolizing enzyme peptides of the present invention. The present invention specifically provides isolated peptide and nucleic acid molecules, methods of identifying orthologs and paralogs of the drug-metabolizing enzyme peptides, and methods of identifying modulators of the drug-metabolizing enzyme peptides.
1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence that encodes a protein comprising the amino acid sequence of SEQ ID NO:2;
(b) a nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID No: 1;
(c) a nucleic acid molecule consisting of the nucleic acid sequence of SEQ ID No: 3; and
(d) a nucleotide sequence that is completely complementary to a nucleotide sequence of (a)-(c).
2. A nucleic acid vector comprising a nucleic acid molecule of claim 1 .
3. A host cell containing the vector of claim 2 .
4. A process for producing a polypeptide comprising culturing the host cell of claim 3 under conditions sufficient for the production of said polypeptide, and recovering the peptide from the host cell culture.
5. An isolated polynucleotide comprising a nucleotide sequence set forth in SEQ ID NO:1.
6. An isolated polynucleotide comprising a nucleotide sequence set forth in SEQ ID NO:3.
7. A vector according to claim 2 , wherein said vector is selected from the group consisting of a plasmid, virus, and bacteriophage.
8. A vector according to claim 2 , wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that the protein of SEQ ID NO: 2 may be expressed by a cell transformed with said vector.
9. A vector according to claim 8 , wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.
10. An isolated nucleic acid molecule encoding a human drug-metabolizing enzyme peptide, said nucleic acid molecule sharing at least 95 percent homology with a nucleic acid molecule shown in SEQ ID NOS:1 or 3.
11. A nucleic acid vector comprising a nucleic acid molecule of claim 10 .
12. A host cell containing the vector of claim 11 .
13. A process for producing a polypeptide comprising culturing the host cell of claim 12 under conditions sufficient for the production of said polypeptide, and recovering the peptide from the host cell culture.
14. A vector according to claim 11 , wherein said vector is selected from the group consisting of a plasmid, virus, and bacteriophage.
15. A vector according to claim 11 , wherein said isolated nucleic acid molecule is inserted into said vector in proper orientation and correct reading frame such that the protein of SEQ ID NO: 2 may be expressed by a cell transformed with said vector.
16. A vector according to claim 15 , wherein said isolated nucleic acid molecule is operatively linked to a promoter sequence.