High viscosity xanthan polymer preparations
View Patent ↗Increasing the molecular length of xanthan polymer makes a higher viscosity xanthan composition. Xanthan with higher specific viscosity characteristics provides more viscosity at equivalent concentration in food, industrial and oilfield applications. Methods for increasing the viscosity of xanthan include inducing particular key genes and increasing copy number of particular key genes.
1. A method of producing a xanthan composition comprising a population of xanthan molecules having a range of molecular lengths wherein at least 5% of the population has a length of at least 3 um as measured by atomic force microscopy, comprising: selectively increasing the amount of gene product of wild type gumB and wild type gum C, in a Xanthomonas campestris culture, wherein multiple copies of said wild type gumB and wild type gumC are present but without multiple copes of orfX, gumD, gumE, gumF, and gumG and culturing said Xanthomonas campestris strain.
2. A method of producing a xanthan polymer preparation having increased viscosity relative to that produced by a strain not having amplified genes gumB and gumC, comprising:
selectively increasing the amount of gene product of wild-type gumB and the gene product of wild-type gumC, but not selectively increasing the amount of gene product of orfX and not selectively increasing the amount of the gene products of gumD, gumE, gumF and gumG in said Xanthomonas campestris strain and culturing said Xanthomonas campestris strain, whereby a higher viscosity xanthan polymer preparation is produced by the culture.
3. The method of claim 2 wherein the step of selectively increasing the amount of gene product of gumB and gumC is performed by introducing into the Xanthomonas campestris strain one or more additional copies of gumB and gumC.
4. The method of claim 3 wherein the additional copies are on an extrachromosomal genetic element.
5. The method of claim 4 wherein the extrachromosomal genetic element is a plasmid.
6. The method of claim 5 wherein the plasmid is a broad host range plasmid.
7. The method of claim 2 wherein the additional copies are integrated in the genome of the Xanthomonas campestris strain.
8. The method of claim 2 wherein the step of selectively increasing the amount of gene product of gumB and gumC is performed by inducing gumB and gumC expression using an inducible promoter and an inducing agent which increases expression from the inducible promoter.
9. The method of claim 2 further comprising the step of recovering the higher viscosity xanthan polymer from the preparation.
10. The method of claim 2 further comprising the step of precipitating xanthan polymer from the higher viscosity xanthan polymer preparation.
11. The method of claim 1 wherein the Xanthomonas campestris strain comprises one or more plasmids which in aggregate comprise at least one copy of gumB and gumC.
12. The method of claim 1 further comprising the step of recovering a higher viscosity xanthan polymer from the culture medium.
13. The method of claim 1 further comprising the step of precipitating xanthan polymer from the culture medium.