L-Lysine-producing corynebacteria and process for the preparation of L-lysine
View Patent ↗The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
1. A vector comprising the nucleotide sequence as set forth in SEQ ID NO: 5.
2. A host cell comprising the vector of claim 1 .
3. The host cell of claim 2 that is a prokaryotic cell.
4. A vector comprising the nucleotide sequence as set forth in SEQ ID NO: 5 that is linked to a Corynebacterium dapA gene.
5. A host cell comprising the vector of claim 4 .
6. The host cell of claim 5 , further comprising a vector comprising a Cornybacterium lysC gene.
7. A vector comprising the nucleotide sequence as set forth in SEQ ID NO: 6.
8. A host cell comprising the vector of claim 7 .
9. The host cell of claim 8 that is a prokaryotic cell.
10. A vector comprising the nucleotide sequence as set forth in SEQ ID NO: 6 that is operatively linked to a Corynebacterium dapA gene.
11. A host cell comprising the vector of claim 10 .
12. The host cell of claim 11 , further comprising a vector comprising a Cornybacterium lysC gene.
13. A process of producing L-lysine comprising culturing the host cell of claim 3 under suitable conditions to produce L-lysine, and isolating the L-lysine from the culture.
14. A process of producing L-lysine comprising culturing the host cell of claim 6 under suitable conditions to produce L-lysine, and isolating the L-lysine from the culture.
15. The process of claim 13 wherein the nucleic acid molecule comprises promoter DNA other than the promoter DNA for the native DapA polypeptide operatively linked to the DNA encoding the DapA polypeptide.
16. The process of claim 14 wherein the nucleic acid molecule comprises promoter DNA other than the promoter DNA for the native DapA polypeptide operatively linked to the DNA encoding the DapA polypeptide.
17. A process of producing L-lysine comprising culturing the host cell of claim 9 under suitable conditions to produce L-lysine, and isolating the L-lysine from the culture.
18. A process of producing L-lysine comprising culturing the host cell of claim 12 under suitable conditions to produce L-lysine, and isolating the L-lysine from the culture.
19. The process of claim 17 wherein the nucleic acid molecule comprises promoter DNA other than the promoter DNA for the native DapA polypeptide operatively linked to the DNA encoding the DapA polypeptide.
20. The process of claim 18 wherein the nucleic acid molecule comprises promoter DNA other than the promoter DNA for the native DapA polypeptide operatively linked to the DNA encoding the DapA polypeptide.