Plant promoters for use in early seed development
View Patent ↗The present invention relates to DNA molecules that encode transcription regulatory regions. Furthermore, this present invention relates to nucleotide sequences encoding transcription regulatory regions that promote early seed enhanced or seed coat enhanced transcription of contiguous nucleotide sequences.
1. A promoter comprising an isolated polynucleotide sequence selected from the group of polynucleotide sequences consisting of:
a) a polynucleotide sequence comprising the sequence of SEQ ID NO:4; and;
b) a polynucleotide sequence comprising a fragment of the sequence of SEQ ID NO:4 with promoter function;
wherein the promoter is operably linked to a transcribable polynucleotide molecule.
2. A construct comprising the promoter of claim 1 , wherein said promoter is operably linked to a transcribable polynucleotide molecule operably linked to a 3′ transcription termination polynucleotide molecule.
3. The construct of claim 2 , wherein said transcribable polynucleotide molecule is a gene of agronomic interest.
4. The construct of claim 2 , wherein said transcribable polynucleotide molecule is a marker gene.
5. A transgenic, seed-producing dicotyledonous plant stably transformed with a construct comprising the promoter of claim 1 , wherein said promoter is operably linked to a transcribable polynucleotide molecule operably linked to a 3′ transcription termination polynucleotide molecule.
6. The transgenic dicotyledonous plant of claim 5 , wherein said plant is a dicotyledonous plant selected from the group consisting of tobacco, tomato, potato, peanut, soybean, cotton, canola, rapeseed, safflower, flax, sugarbeet, Arabidopsis, Brassica , sunflower, and alfalfa.
7. The transgenic dicotyledonous plant of claim 5 , wherein said transcribable polynucleotide molecule confers altered oil content in the seed to said transgenic plant.
8. The transgenic dicotyledonous plant of claim 5 , wherein said transcribable polynucleotide molecule confers altered protein quality in the seed to said transgenic plant.
9. The transgenic dicotyledonous plant of claim 5 , wherein said transcribable polynucleotide molecule confers altered micronutrient content in the seed to said transgenic plant.
10. A seed of said transgenic plant of claim 5 , wherein the seed comprises said construct.
11. Meal from said transgenic plant of claim 5 .
12. A method of making a vegetable oil and meal, comprising the steps of: a) incorporating in the genome of a dicotyledonous seed producing, oil-containing plant a promoter according to claim 1 operably linked to a transcribable polynucleotide molecule conferring altered oil content; b) growing the dicotyledonous plant to produce seeds; and c) extracting oil from the seed to produce extracted oil and meal.