Nucleic acid molecules encoding enzymes from wheat which are involved in starch synthesis
View Patent ↗The present invention relates to nucleic acid molecules encoding enzymes which are involved in the starch synthesis in plants. These enzymes are starch synthases from wheat. The invention further relates to vectors and host cells containing said nucleic acid molecules, in particular transformed plant cells and plants regenerated from these cells, which exhibit an increased or a reduced activity of the described starch synthases.
1. Starch obtainable from a plant cell produced by a method comprising the step of introducing into said plant cell a nucleic acid molecule comprising a regulatory element operably linked to a part of a nucleic acid sequence in sense orientation thereto, wherein the nucleic acid sequence is selected from the group consisting of:
(a) a nucleic acid sequence encoding a protein that has the amino acid sequence of SEQ ID NO: 6;
(b) a nucleic acid sequence that is SEQ ID NO: 5; and
(c) a nucleic acid sequence that has more than 90% sequence identity to the coding region of SEQ ID NO: 5;
wherein the nucleic acid sequence encodes a protein with the biological activity of a soluble starch synthase.
2. Starch obtainable from a plant cell produced by a method comprising the step of introducing into said plant cell a nucleic acid molecule comprising a regulatory element operably linked to a part of a nucleic acid sequence in antisense or sense orientation thereto, wherein the nucleic acid sequence is selected from the group consisting of:
(a) a nucleic acid sequence encoding a protein that has the amino acid sequence of SEQ ID NO: 6;
(b) a nucleic acid sequence that is SEQ ID NO: 5; and
(c) a nucleic acid sequence that has more than 90% sequence identity to the coding region of SEQ ID NO: 5;
wherein the nucleic acid molecule is sufficient to reduce the expression of a starch synthase in said plant cell.
3. The starch of claim 1 or 2 , wherein the starch has modified chemical and/or physical properties.
4. The starch of claim 1 or 2 , wherein the nucleic acid sequence has between 95% and 100% sequence identity to the coding region of SEQ ID NO: 5.
5. The starch of claim 1 or 2 , wherein the plant cell is from a starch-storing and/or starch-synthesizing plant.
6. The starch of claim 5 , wherein the starch-storing and/or starch-synthesizing plant is selected from the group consisting of rye, barley, oats, wheat, rice, maize, peas, cassava and potatoes.
7. The starch of claim 6 , wherein the plant is a wheat plant.