Method of hydrolyzing jojoba protein
View Patent ↗Unhydrolyzed and hydrolyzed jojoba protein having high simmondsin concentration are provided. These jojoba proteins may be in the form of an aqueous dispersion containing a mixture of amino acids, peptides, and/or protein fractions derived from the extraction and hydrolysis of naturally occurring jojoba protein, or dried into a powder.
1. A method of hydrolyzing jojoba protein extracted from defatted jojoba meal comprising the steps of:
forming an aqueous dispersion of said defatted jojoba meal followed by
filtering said aqueous dispersion thereby producing a permeate comprising unhydrolyzed jojoba protein followed by
hydrolyzing said unhydrolyzed jojoba protein by adding protease enzymes to said permeate and agitating said permeate to form an agitated dispersion followed by
deactivating the protease enzymes in said dispersion
said dispersion, following said enzyme deactivation step, comprising from about 5–15% by weight simmondsin on a dry basis.
2. The method of claim 1 farther comprising the step of adjusting the pH of the permeate prior to the addition of protease enzymes thereto.
3. The method of claim 1 including the step of passing said dispersion after said enzyme deactivation step through a filtration system to generate respective hydrolyzed jojoba amino acid permeate and hydrolyzed jojoba protein retentate fractions having different molecular weight profiles, with the retentate fraction having a higher molecular weight profile than said permeate fraction.
4. The method of claim 3 including the step of drying said hydrolyzed jojoba protein retentate into a hydrolyzed jojoba protein powder.
5. The method of claim 4 , said drying step comprising spray drying said hydrolyzed jojoba protein retentate.
6. The method of claim 4 , wherein said hydrolyzed jojoba protein powder comprises from about 18–35% by weight protein.
7. The method of claim 6 , wherein said hydrolyzed jojoba protein having a molecular weight range of from about 1,000–5,000.
8. The method of claim 3 , including the step of drying said hydrolyzed jojoba amino acid permeate into a hydrolyzed jojoba amino acid powder.
9. The method of claim 8 , said drying step comprising spray drying said hydrolyzed jojoba amino acid permeate.
10. The method of claim 3 , wherein said hydrolyzed jojoba amino acid powder comprises from about 3–15% by weight amino acid.
11. The method of claim 10 , wherein said hydrolyzed jojoba amino acid having an average molecular weight from about 75–1,000.
12. The method of claim 3 , wherein said hydrolyzed jojoba amino acid powder comprises from about 1–3% by weight simmondsin.
13. The method of claim 1 including the step of heating said aqueous dispersion to a temperature between about 60–90° C.