Feline IL-12 single chain nucleic acid molecules
View Patent ↗The present invention relates to canine and feline proteins. In particular, the present invention discloses feline interleukin-18, feline caspase-1, feline interleukin-12 single chain and canine interleukin-12 single chain proteins. The present invention also includes feline interleukin-18, feline caspase-1, feline interleukin-12 single chain and canine interleukin-12 single chain nucleic acid molecules encoding such proteins, antibodies raised against such proteins and/or inhibitors of such proteins or nucleic acid molecules. The present invention also includes therapeutic compositions comprising such nucleic acid molecules, proteins, antibodies and/or inhibitors, as well as their use to evaluate and regulate an immune response in an animal.
1. An isolated nucleic acid molecule selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising:
(i) a nucleic acid sequence encoding a feline p35 subunit protein;
(ii) a nucleic acid linker of (XXX) n wherein n=0 to 60; and
(iii) a nucleic acid sequence encoding a feline p40 subunit protein; and
(b) an isolated nucleic acid molecule comprising a nucleic acid sequence fully complementary to the nucleic acid molecule set forth in (a).
2. The isolated nucleic acid molecule of claim 1 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p35 subunit encoding nucleic acid sequence comprises at least 44 contiguous nucleotides identical in sequence to at least 44 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID NO:32, SEQ ID NO:35 and SEQ ID NO:101; and
(b) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p40 subunit encoding nucleic acid sequence comprises at least 44 contiguous nucleotides identical in sequence to at least 44 contiguous nucleotides of a nucleic acid sequence selected from the group consisting of SEQ ID NO:26, SEQ ID NO:29 and SEQ ID NO:55.
3. The isolated nucleic acid molecule of claim 1 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p35 subunit encoding nucleic acid sequence comprises a nucleotide sequence at least 90% identical to SEQ ID NO:32, SEQ ID NO:35 and SEQ ID NO:101;
(b) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p40 subunit encoding nucleic acid sequence comprises a nucleotide sequence at least 90% identical to SEQ ID NO:26, SEQ ID NO:29 and SEQ ID NO:55; and
(c) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said nucleic acid molecule comprises a nucleic acid sequence at least 90% identical to SEQ ID NO:38 or SEQ ID NO:43.
4. The isolated nucleic acid molecule of claim 1 , wherein said nucleic acid molecule encodes a protein having a function selected from the group consisting of:
(a) eliciting an immune response against an IL-12 protein having the amino acid sequence of SEQ ID NO: 39 or SEQ ID NO: 44;
(b) selectively binding to an antibody raised against an IL-12 protein having the amino acid sequence of SEQ ID NO: 39 or SEQ ID NO: 44; and
(c) exhibiting IL-12 activity.
5. The isolated nucleic acid molecule of claim 1 , wherein said nucleic acid linker comprises SEQ ID NO:83.
6. The isolated nucleic acid molecule of claim 1 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p35 subunit encoding nucleic acid sequence comprises SEQ ID NO:32, SEQ ID NO:35 or SEQ ID NO:101;
(b) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p40 subunit encoding nucleic acid sequence comprises SEQ ID NO:26, SEQ ID NO:29 or SEQ ID NO:55; and
(c) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said isolated nucleic acid molecule comprises SEQ ID NO:38 or SEQ ID NO:43.
7. The isolated nucleic acid molecule of claim 1 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p35 subunit encoding nucleic acid sequence consists of SEQ ID NO:32, SEQ ID NO:35 or SEQ ID NO:101;
(b) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said p40 subunit encoding nucleic acid sequence consists of SEQ ID NO:26, SEQ ID NO:29 or SEQ ID NO:55; and
(c) an isolated nucleic acid molecule comprising a feline p35 subunit encoding sequence and a feline p40 subunit encoding sequence, wherein said isolated nucleic acid molecule has a nucleic acid sequence consisting of SEQ ID NO:38 or SEQ ID NO:43.
8. An isolated nucleic acid molecule selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising:
(i) a first nucleic acid sequence encoding a protein comprising an at least 23 contiguous amino acid region identical in sequence to an at least 23 contiguous amino acid region from SEQ ID NO:33, SEQ ID NO:36 or SEQ ID NO:102;
(ii) a nucleic acid linker of (XXX)n wherein n=0 to 60; and
(iii) a second nucleic acid sequence encoding a protein comprising an at least 23 contiguous amino acid region identical in sequence to an at least 23 contiguous amino acid region from SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:56; and
(b) an isolated nucleic acid molecule comprising a nucleic acid sequence fully complementary to the nucleic acid molecule set forth in (a).
9. The isolated nucleic acid molecule of claim 8 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said first nucleic acid sequence encodes a protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:33, SEQ ID NO:36 or SEQ ID NO:102;
(b) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said second nucleic acid sequence encodes a protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:56; and
(c) an isolated nucleic acid molecule encoding a protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:39 or SEQ ID NO:44.
10. The isolated nucleic acid molecule of claim 8 , wherein said nucleic acid molecule encodes a protein having a function selected from the group consisting of:
(a) eliciting an immune response against an IL-12 protein having the amino acid sequence of SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:56;
(b) selectively binding to an antibody raised against an IL-12 protein having the amino acid sequence of SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO:56; and
(c) exhibiting IL-12 activity.
11. The isolated nucleic acid molecule of claim 8 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said first nucleic acid sequence encodes an amino acid sequence comprising SEQ ID NO:33, SEQ ID NO:36 or SEQ ID NO: 102;
(b) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said second nucleic acid sequence encodes an amino acid sequence comprising SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO: 56; and
(c) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said isolated nucleic acid sequence encodes a protein comprising SEQ ID NO:39 or SEQ ID NO:44.
12. The isolated nucleic acid molecule of claim 8 , wherein said isolated nucleic acid molecule is selected from the group consisting of:
(a) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said first nucleic acid sequence encodes an amino acid sequence consisting of SEQ ID NO:33, SEQ ID NO:36 or SEQ ID NO: 102;
(b) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said second nucleic acid sequence encodes an amino acid sequence consisting of SEQ ID NO:27, SEQ ID NO:30 or SEQ ID NO: 56; and
(c) an isolated nucleic acid molecule comprising a first and second nucleic acid sequence, wherein said isolated nucleic acid sequence encodes a protein having the sequence of SEQ ID NO:39 or SEQ ID NO:44.
13. The isolated nucleic acid molecule of claim 8 , wherein said nucleic acid linker comprises SEQ ID NO:83.
14. A recombinant virus comprising a nucleic acid molecule as set forth in claim 1 .
15. A recombinant cell comprising a nucleic acid molecule as set forth in claim 1 .
16. A kit comprising a nucleic acid molecule as set forth in claim 1 .
17. A recombinant virus comprising a nucleic acid molecule as set forth in claim 8 .
18. A recombinant cell comprising a nucleic acid molecule as set forth in claim 8 .
19. A kit comprising a nucleic acid molecule as set forth in claim 8 .