IP Library Granted Patent US 8,048,867
Granted Patent B2
US 8,048,867 · App. 10/839,023 · Granted Nov 1, 2011

9-substituted minocycline compounds

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Quick Facts
Patent No.
US 8,048,867
App. No.
10/839,023
Granted
Nov 1, 2011
Kind
B2
Abstract

The present invention pertains, at least in part, to novel 9-substituted minocycline compounds. These minocycline compounds can be used to treat numerous tetracycline compound-responsive states, such as bacterial infections and neoplasms, as well as other known applications for minocycline and tetracycline compounds in general, such as blocking tetracycline efflux and modulation of gene expression.

Claims (83)

1. A minocycline compound of formula I:

wherein:

X is CHC(R 13 Y′Y) or CR 6 R 6′ ;

R 2 , R 4′ and R 4″ are each independently hydrogen, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, aryl, heterocyclic, or heteroaromatic;

R 7′ and R 7″ are each independently alkyl;

R 4 is NR 4′ R 4″ , alkyl, alkenyl, alkynyl, aryl, hydroxyl, or halogen;

R 2′ , R 3 , R 10 , R 11 and R 12 are each hydrogen;

R 5 is hydroxyl, hydrogen, thiol, alkanoyl, aroyl, alkaroyl, aryl, heteroaromatic, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, arylalkyl, alkyl carbonyloxy, or aryl carbonyloxy;

R 6 and R 6′ are each independently hydrogen, hydroxyl, halogen, thiol, alkyl, alkenyl, alkynyl, aryl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or arylalkyl;

R 9 is an unsubstituted single-ring aromatic group having zero heteroatoms or a single-ring aromatic group having zero heteroatoms substituted with alkyl, alkenyl, alkynyl, aryl, halogen, hydroxyl, alkoxy, formyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylaminoacarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, phosphate, phosphonato, phosphinato, amino, alkylamino, dialkylamino, arylamino, diarylamino, alkylarylamino, acylamino, alkylcarbonylamino, arylcarbonylamino, carbamoyl, ureido, amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfate, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, aryl or a heteroaryl;

R 8 is hydrogen, hydroxyl, halogen, thiol, alkyl, alkenyl, alkynyl, aryl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or arylalkyl;

R 13 is hydrogen, hydroxy, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or arylalkyl; and

Y′ and Y are each independently hydrogen, halogen, hydroxyl, cyano, sulfhydryl, amino, alkyl, alkenyl, alkynyl, alkoxy, alkylthio, alkylsulfinyl, alkylsulfonyl, alkylamino, or arylalkyl, or pharmaceutically acceptable salts or esters thereof.

2. The minocycline compound of claim 1 , wherein R 4 is NR 4′ R 4″ ; X is CR 6 R 6′ ; R 2 , R 5 , R 6 , R 6′ and R 8 , are each hydrogen; and R 4′ , R 4″ , R 7′ and R 7″ are each lower alkyl.

3. The minocycline compound of claim 2 , wherein R 4′ , R 4″ , R 7′ , and R 7″ are each methyl.

4. The minocycline compound of claim 1 , wherein R 9 is unsubstituted phenyl.

5. The minocycline compound of claim 1 , wherein R 9 is phenyl substituted with one or more substituents selected from the group consisting of alkyl, alkenyl, alkynyl, aryl, halogen, hydroxyl, alkoxy, formyl, alkylcarbonyloxy, arylcarbonyloxy, carboxyl, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylaminocarbonyl, arylalkyl aminocarbonyl, alkenylaminocarbonyl, alkylcarbonyl, arylcarbonyl, arylalkylcarbonyl, alkenylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylthiocarbonyl, phosphate, phosphonato, phosphinato, amino, acylamino, amido, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, aryl and a heteroaryl.

6. The minocycline compound of claim 5 , wherein said phenyl is substituted with one or more substituents selected from the group consisting of carboxylate, alkyl, alkenyl, alkynyl, aryl, heterocyclic, cyano, amino, halogen, alkoxy, alkoxycarbonyl, amido, alkylcarbonyl, and nitro.

7. A method for treating a bacterial infection in a subject, comprising administering to said subject aminocycline compound of claim 1 , such that said subject is treated, wherein said bacterial infection is caused by a gram-positive bacterium or a gram-negative bacterium and said gram-positive bacterium is selected from the group consisting of S. aureus, E. hirae and E. faecalis and said gram-negative bacterium is selected from the group consisting of E. coli, K. pneumoniae, Salmonella, A. baumanii, B. catarrhalis and P. aeruginosa.

8. The method of claim 7 , wherein said bacterial infection is caused by E. coli.

9. The method of claim 7 , wherein said bacterial infection is caused by S. aureus.

10. The method of claim 7 , wherein said bacterial infection is caused by E. faecalis.

11. The method of claim 7 , wherein said minocycline compound is administered with a pharmaceutically acceptable carrier.

12. The method of claim 7 , wherein said subject is a human.

13. A pharmaceutical composition comprising a therapeutically effective amount of aminocycline compound of claim 1 and a pharmaceutically acceptable carrier.

14. A minocycline compound of the formula

or a pharmaceutically acceptable salt thereof.

15. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

16. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

17. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

18. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

19. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

20. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

21. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

22. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

23. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

24. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

25. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

26. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

27. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

28. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

29. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

30. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

31. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

32. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

33. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

34. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

35. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

36. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

37. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

38. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

39. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

40. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

41. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

42. The minocycline compound of claim 1 , wherein said compound is:

or a pharmaceutically acceptable salt thereof.

Assignments (12)
TERMINATION OF LIEN ON PATENTS Recorded Dec 23, 2014
From: MINTZ LEVIN COHN FERRIS GLOVSKY AND POPEO PC
To: PARATEK PHARMACEUTICALS, INC.
Reel/Frame 034700/0377 →
RELEASE OF SECURITY INTEREST Recorded Oct 31, 2014
From: HBM HEALTHCARE INVESTMENTS (CAYMAN) LTD., AS COLLATERAL AGENT
To: PARATEK PHARMACEUTICALS, INC.
Reel/Frame 034113/0910 →
SECURITY INTEREST Recorded Mar 14, 2014
From: PARATEK PHARMACEUTICALS, INC.
To: HBM HEALTHCARE INVESTMENTS (CAYMAN) LTD., AS COLLATERAL AGENT
Reel/Frame 032448/0001 →
NOTICE Recorded Mar 8, 2013
From: PARATEK PHARMACEUTICALS, INC.
To: MINTZ LEVIN COHN FERRIS GLOVSKY AND POPEO PC
Reel/Frame 029940/0106 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2011
From: FRECHETTE, ROGER; VISKI, PETER; BOWSER, TODD E.; STAPLETON, KAREN; HAWKINS, PAUL; BHATIA, BEENA; VERMA, ATUL; MCINTYRE, LAURA; WARCHOL, TADEUSZ
To: PARATEK PHARMACEUTICALS, INC.
Reel/Frame 026826/0957 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2011
From: MESSERSMITH, DAVID
To: PARATEK PHARMACEUTICALS, INC.
Reel/Frame 026826/0978 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2011
From: DUMORNAY, JIMMY; LIU, GUI; SHEAHAN, PAUL
To: TRUSTEES OF TUFTS COLLEGE
Reel/Frame 026826/0924 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2011
From: RENNIE, GLEN
To: TRUSTEES OF TUFTS COLLEGE
Reel/Frame 026826/0889 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2011
From: KOZA, DARRELL
To: TRUSTEES OF TUFTS COLLEGE
Reel/Frame 026826/0915 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 30, 2011
From: NELSON, MARK L.; ISMAIL, MOHAMED Y.
To: TRUSTEES OF TUFTS COLLEGE; PARATEK PHARMACEUTICALS, INC.
Reel/Frame 026826/0934 →
RELEASE OF SECURITY INTEREST Recorded Oct 9, 2009
From: MIDCAP FINANCIAL, LLC
To: PARATEK PHARMACEUTICALS, INC.
Reel/Frame 023348/0621 →
SECURITY AGREEMENT Recorded Jul 6, 2009
From: PARATEK PHARMACEUTICALS, INC.
To: MIDCAP FINANCIAL, LLC
Reel/Frame 022917/0112 →