Methods and apparatus for synthesizing labeled combinatorial chemistry libraries
View Patent ↗The present invention provides labeled synthetic libraries of random oligomers and methods and apparatus for generating labeled synthetic oligomer libraries. Each member of such a library is labeled with a unique identifier tag that specifies the structure or sequence of the oligomer. In a preferred embodiment of the present invention the identifier tag is a microchip that is pre-encoded or encodable with information that is related back to a detector when the identifier tag is pulsed with electromagnetic radiation.
1. A pre-encoded substrate useful for being linked to a single oligomer structure whereby said pre-encoded substrate bears a unique identifier tag, said tag being a pre-encoded microchip identifying said oligomer structure.
2. An encodable substrate useful for being linked to a single oligomer structure whereby said encodable substrate has a unique identifier tag, said tag being a encodable microchip identifying said oligomer structure.
3. One or a plurality of the pre-encoded substrate of claim 1 useful for in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct pre-encoded substrate whereby said one or plurality of pre-encoded substrate is linked to a oligomer structure said method comprising the steps of:
a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
b) exposing said pre-encoded substrates in each reaction vessel to one or a plurality of transformation events;
c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times wherein said preencoded substrate being a pre-encoded microchip.
4. One or a plurality of the encodable substrate of claim 2 useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct encodable substrate whereby said one or plurality of pre-encoded substrate a oligomer structure is linked to a oligomer structure said method comprising the steps of:
a) apportioning said encodable substrates among a plurality of reaction vessels;
b) exposing said encodable substrates in each reaction vessels to one or a plurality of transformation events;
c) adding first identifier information to said encodable substrates;
d) apportioning said encodable substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times wherein said encodable substrate being a encodable microchip.
5. One or a plurality of the pre-encoded substrate of claim 1 useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct pre-encoded substrate whereby said one or plurality of pre-encoded substrate is linked to a oligomer structure said method comprising the steps of:
a) apportioning said pre-encoded substrates among a plurality of reaction vessels;
b) exposing said pre-encoded substrates in each reaction vessels to one or a plurality of monomers;
c) detecting and recording identifier information for each of said identifier tags in each of said reaction vessels;
d) apportioning said pre-encoded substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times wherein said pre-encoded substrate being a pre-encoded microchip.
6. One or a plurality of the encodable substrate of claim 2 useful in a method of preparing a labeled synthetic oligomer library comprising a plurality of different members, each member comprising a distinct encodable substrate whereby said one or plurality of pre-encoded substrate a oligomer structure is linked to a oligomer structure said method comprising the steps of:
a) apportioning said encodable substrates among a plurality of reaction vessels;
b) exposing said encodable substrates in each reaction vessels to one or a plurality of monomers;
c) adding first identifier information to said encodable substrates;
d) apportioning said encodable substrates among a plurality of reaction vessels; and
e) repeating steps a) through c) from at least one to about twenty times wherein said encodable substrate being a encodable microchip.