Electromagnetic fields increase in vitro and in vivo angiogenesis through endothelial release of FGF-2
The present invention relates to a method of inducing angiogenesis in a cell or tissue by applying an electromagnetic field to the cell or tissue under conditions effective to induce angiogenesis. Also disclosed is a method of treating an ischemic condition in a patient by applying an electromagnetic field to ischemic tissue in a patient under conditions effective to treat the ischemic condition by inducing angiogenesis. A method of tissue engineering is also disclosed. This method involves providing a tissue scaffold and subjecting the tissue scaffold to an electromagnetic field under conditions effective to form a vascularized tissue scaffold. Further disclosed is a method of inducing activity of angiogenic growth factors by applying an electromagnetic field to a cell or tissue under conditions effective to induce activity of an angiogenic growth factor.
1 . A method of inducing angiogenesis in a cell or tissue, said method comprising:
applying an electromagnetic field to the cell or tissue under conditions effective to induce angiogenesis.
2 . The method according to claim 1 , wherein the cell is an endothelial cell or the tissue comprises endothelial cells.
3 . The method according to claim 1 , wherein the electromagnetic field comprises pulsed electromagnetic fields.
4 . The method according to claim 1 , wherein said applying an electromagnetic field is carried out with a device capable of emitting an electromagnetic field.
5 . The method according to claim 4 , wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which operates at a frequency of about 5 to 25 Hz.
6 . The method according to claim 4 , wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which produces asymmetric pulses lasting for about 1 to 10 msec.
7 . A method of treating an ischemic condition in a patient, said method comprising:
applying an electromagnetic field to ischemic tissue in a patient under conditions effective to treat the ischemic condition by inducing angiogenesis.
8 . The method according to claim 7 , wherein the electromagnetic field comprises pulsed electromagnetic fields.
9 . The method according to claim 7 , wherein the ischemic condition is coronary artery disease.
10 . The method according to claim 7 , wherein the ischemic condition is peripheral vascular disease.
11 . The method according to claim 7 , wherein the ischemic condition is cerebrovascular disease.
12 . The method according to claim 7 , wherein the ischemic condition is a wound.
13 . The method according to claim 12 , wherein the wound is a chronic wound.
14 . The method according to claim 12 , wherein the wound is a diabetic wound.
15 . The method according to claim 7 , wherein the electromagnetic field is applied to surgically exposed tissue.
16 . The method according to claim 7 , wherein the electromagnetic field is applied to skin overlying tissue.
17 . The method according to claim 7 , wherein the ischemic tissue is positioned within an electromagnetic field device.
18 . The method according to claim 7 , wherein said applying comprises:
providing a device capable of emitting an electromagnetic field, and subjecting the ischemic tissue to an electromagnetic field with said device.
19 . The method according to claim 18 , wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which operates at a frequency of about 5 to 25 Hz.
20 . The method according to claim 18 , wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which produces asymmetric pulses lasting for about 1 to 10 msec.
21 . The method according to claim 7 , wherein the patient is human.
22 . A method of tissue engineering comprising:
providing a tissue scaffold, and subjecting the tissue scaffold to an electromagnetic field under conditions effective to form a vascularized tissue scaffold.
23 . The method according to claim 22 , wherein the electromagnetic field comprises pulsed electromagnetic fields.
24 . The method according to claim 22 , wherein said subjecting is carried out in vivo.
25 . A method of inducing activity of angiogenic growth factors, said method comprising:
applying an electromagnetic field to a cell or tissue under conditions effective to induce activity of an angiogenic growth factor.
26 . The method according to claim 25 , wherein the electromagnetic field comprises pulsed electromagnetic fields.
27 . The method according to claim 25 , wherein said applying comprises:
applying the electromagnetic field with a device capable of emitting an electromagnetic field.
28 . The method according to claim 27 , wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which operates at a frequency of about 5 to 25 Hz.
29 . The method according to claim 27 , wherein the device capable of emitting an electromagnetic field is a pulsed electromagnetic field device which produces asymmetric pulses lasting for about 1 to 10 msec.
30 . The method according to claim 25 , wherein the angiogenic growth factor is Fibroblast Growth Factor 2.