Compositions and methods for detecting
View Patent ↗The present invention relates to oligonucleotides useful for determining the presence of Trichomonas vaginalis in a test sample. The oligonucleotides of the present invention may be incorporated into detection probes, helper probes, capture probes and amplification oligonucleotides, and used in various combinations thereof.
1. A detection probe for use in determining the presence of Trichomonas vaginalis in a test sample, said probe comprising a target binding region consisting of the base sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, wherein said probe forms a hybrid stable for detection with nucleic acid derived from Trichomonas vaginalis but not from Trichomonas tenax under assay conditions which include a temperature of about 60° C. and a salt concentration of about 0.6 M to about 0.9 M, and wherein said probe does not include bases in addition to the bases of said target binding region which participate in stable hybridization with Trichomonas vaginalis derived nucleic acid under said assay conditions.
2. The probe of claim 1 , wherein the base sequence of said probe consists of the base sequence of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4.
3. The probe of claim 1 , wherein said probe is a self-hybridizing probe under said assay conditions and in the absence of nucleic acid derived from Trichomonas vaginalis.
4. The probe of claim 3 , wherein said probe comprises a pair of interacting labels.
5. The probe of claim 1 , wherein said probe is up to 50 bases in length.
6. The probe of claim 1 , wherein said probe comprises a detectable label.
7. The probe of claim 1 , wherein said target binding region includes at least one ribonucleotide modified to include a 2′-O-methyl substitution to the ribofuranosyl moiety or a pseudo peptide backbone joins at least a portion of the bases of said target binding region.
8. A composition comprising said probe of claim 1 hybridized to nucleic acid derived from Trichomonas vaginalis.
9. A probe mix comprising said probe of claim 1 and a helper probe.
10. The probe mix of claim 9 , wherein the base sequence of said helper probe consists of the base sequence of SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27 or SEQ ID NO:28.
11. A method for determining the presence of Trichomonas vaginalis , said method comprising the steps of:
a) contacting a test sample with said probe of claim 1 ; and
b) determining whether said hybrid has formed as indication of the presence of Trichomonas vaginalis in said test sample.
12. A detection probe for use in determining the presence of Trichomonas vaginalis in a test sample, said probe comprising a target binding region consisting of or contained within the base sequence of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, wherein said probe forms a hybrid stable for detection with nucleic acid derived from Trichomonas vaginalis but not from Trichomonas tenax under assay conditions which include a temperature of about 60° C. and a salt concentration of about 0.6 M to about 0.9 M, and wherein said probe does not include bases in addition to the bases of said target binding region which participate in stable hybridization with Trichomonas vaginalis derived nucleic acid under said assay conditions.
13. The probe of claim 12 , wherein the base sequence of said probe consists of or is contained within the base sequence of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.
14. The probe of claim 12 , wherein the base sequence of said probe consists of the base sequence of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.
15. The probe of claim 12 , wherein said probe is a self-hybridizing probe under said assay conditions and in the absence of nucleic acid derived from Trichomonas vaginalis.
16. The probe of claim 15 , wherein said probe comprises a pair of interacting labels.
17. The probe of claim 12 , wherein said probe is up to 50 bases in length.
18. The probe of claim 12 , wherein said probe comprises a detectable label.
19. The probe of claim 12 , wherein said target binding region includes at least one ribonucleotide modified to include a 2′-O-methyl substitution to the ribofuranosyl moiety or a pseudo peptide backbone joins at least a portion of the bases of said target binding region.
20. A composition comprising said probe of claim 12 hybridized to nucleic acid derived from Trichomonas vaginalis.
21. A method for determining the presence of Trichomonas vaginalis , said method comprising the steps of:
a) contacting a test sample with said probe of claim 12 ; and
b) determining whether said hybrid has formed as indication of the presence of Trichomonas vaginalis in said test sample.
22. A detection probe for use in determining the presence of Trichomonas vaginalis in a test sample, said probe comprising a target binding region consisting of or contained within the base sequence of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 or SEQ ID NO:12, wherein said probe forms a hybrid stable for detection with nucleic acid derived from Trichomonas vaginalis but not from Trichomonas tenax under assay conditions which include a temperature of about 60° C. and a salt concentration of about 0.6 M to about 0.9 M, and wherein said probe does not include bases in addition to the bases of said target binding region which participate in stable hybridization with Trichomonas vaginalis derived nucleic acid under said assay conditions.
23. The probe of claim 22 , wherein the base sequence of said probe consists of or is contained within the base sequence of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 or SEQ ID NO:12.
24. The probe of claim 22 , wherein the base sequence of said probe consists of the base sequence of SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11 or SEQ ID NO:12.
25. The probe of claim 22 , wherein said probe is a self-hybridizing probe under said assay conditions and in the absence of nucleic acid derived from Trichomonas vaginalis.
26. The probe of claim 25 , wherein said probe comprises a pair of interacting labels.
27. The probe of claim 22 , wherein said probe is up to 50 bases in length.
28. The probe of claim 22 , wherein said probe comprises a detectable label.
29. The probe of claim 22 , wherein said target binding region includes at least one ribonucleotide modified to include a 2′-O-methyl substitution to the ribofuranosyl moiety or a pseudo peptide backbone joins at least a portion of the bases of said target binding region.
30. A composition comprising said probe of claim 22 hybridized to nucleic acid derived from Trichomonas vaginalis.
31. A method for determining the presence of Trichomonas vaginalis , said method comprising the steps of:
a) contacting a test sample with said probe of claim 22 ; and
b) determining whether said hybrid has formed as indication of the presence of Trichomonas vaginalis in said test sample.
32. A detection probe for use in determining the presence of Trichomonas vaginalis in a test sample, said probe comprising a target binding region consisting of or contained within the base sequence of SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15 or SEQ ID NO:16, wherein said probe forms a hybrid stable for detection with nucleic acid derived from Trichomonas vaginalis but not from Trichomonas tenax under assay conditions which include a temperature of about 60° C. and a salt concentration of about 0.6 M to about 0.9 M, and wherein said probe does not include bases in addition to the bases of said target binding region which participate in stable hybridization with Trichomonas vaginalis derived nucleic acid under said assay conditions.
33. The probe of claim 32 , wherein the base sequence of said probe consists of or is contained within the base sequence of SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15 or SEQ ID NO:16.
34. The probe of claim 32 , wherein the base sequence of said probe consists of the base sequence of SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15 or SEQ ID NO:16.
35. The probe of claim 32 , wherein said probe is a self-hybridizing probe under said assay conditions and in the absence of nucleic acid derived from Trichomonas vaginalis.
36. The probe of claim 35 , wherein said probe comprises a pair of interacting labels.
37. The probe of claim 32 , wherein said probe is up to 50 bases in length.
38. The probe of claim 32 , wherein said probe comprises a detectable label.
39. The probe of claim 32 , wherein said target binding region includes at least one ribonucleotide modified to include a 2′-O-methyl substitution to the ribofuranosyl moiety or a pseudo peptide backbone joins at least a portion of the bases of said target binding region.
40. A composition comprising said probe of claim 32 hybridized to nucleic acid derived from Trichomonas vaginalis.
41. A method for determining the presence of Trichomonas vaginalis , said method comprising the steps of:
a) contacting a test sample with said probe of claim 32 ; and
b) determining whether said hybrid has formed as indication of the presence of Trichomonas vaginalis in said test sample.