Processing of particles
View Patent ↗Particles dispersed in a liquid are assembled in a configuration in which all the particles lie in the same plane, and the assembled particles are processed while in that configuration. The assembled particles can for example be simultaneously exposed to electromagnetic radiation which elicits from the particles a response which provides information about the particles. The particles can for example be cells, cell fragments, or analyte-bearing beads of the type conventionally analyzed in a cytometer.
1. A method for processing particles comprising the steps of:
A) placing a sample containing said particles dispersed in a liquid into a processing chamber comprising:
i) an inlet;
ii) an elongate particle retention passage (EPRP) having first and second ends, said EPRP being in liquid communication with said inlet;
iii) an elongate particle receptor located between said first and second EPRP ends, said elongate particle receptor comprising:
an elongate barrier member for permitting liquid passage while preventing passage of particles to be processed, said barrier being a weir and the only way in which liquid can pass said barrier member being over said weir; and,
iv) an elongate retention member adjacent said weir and forming an elongate corner with said weir;
B) causing at least some of said particles in said placed sample to be received by said particle receptor so that said received particles are all in the same plane; and,
C) processing at least some of said particles received by said particle receptor.
2. The method of claim 1 wherein step D) comprises removing said particles from said processing chamber so that the processing chamber can be reused.
3. The method of claim 1 wherein substantially all of said particles in said sample are substantially a same size, said size ranging from 0.1 micron to 50 microns.
4. The method of claim 1 wherein the EPRP has a width of about 40 to 100 microns and a depth of 10 to 50 microns.
5. The method of claim 1 wherein said weir and said retention member each include a plurality of pockets, each pocket able to receive only one of said particles.
6. The method of claim 1 wherein step B) further comprises pumping a positioning liquid through said processing chamber to direct said particles toward said particle receptor.
7. The method of claim 1 wherein step B) further comprises flowing said liquid sample to direct said particles towards said particle receptor.
8. The method of claim 1 wherein step C) further comprises simultaneously exposing substantially all of said particles received by said particle receptor to electromagnetic radiation to elicit a response from said received particles that provides information about said received particles.
9. The method of claim 8 wherein substantially all of said received particles are subject to confocal examination.
10. The method of claim 1 wherein step C) further comprises subjecting said received particles to conditions that cause a chemical reaction of the particles.
11. A processing chamber for processing particle-containing liquid samples, said processing chamber comprising:
A) a sample inlet;
B) an elongate particle retention passage (EPRP) having first and second ends, said EPRP in fluid communication with said inlet;
C) an elongate particle receptor located between said first and second ends of said EPRP, said elongate particle receptor comprising:
i) an elongate barrier member, said barrier member being a weir that permits passage of liquid but not passage of particles having a size greater than a selected sized in the range of 1 to 20 microns; and,
ii) an elongate retention member adjacent said weir and forming an elongate corner with said weir, said retention member comprising a plurality of pockets, each pocket having a maximum depth of about 2 to about 20 microns and a width of about 3 to 50 microns.
12. The processing chamber of claim 11 wherein the EPRP has a transverse dimension of 10 to 100 microns.
13. The processing chamber of claim 11 wherein said EPRP has a width of about 40 to about 100 microns and a depth of 10 to 50 microns.
14. A method for processing particles, the method comprising the steps of:
A) providing a processing chamber comprising:
i) an inlet;
ii) an elongate particle retention passage (EPRP) having first and second ends, said EPRP being in fluid communication with said inlet;
iii) an elongate particle receptor located between said first and second EPRP ends comprising an elongate barrier member that permits passage of liquid but not passage of said particles for processing, wherein said barrier member is a weir and the only way that liquid can pass the barrier member is over the weir,
i. wherein the particle receptor comprises an elongate retention member adjacent said weir and forms an elongate corner with said weir, and at least one of said weir and said retention member includes a plurality of pockets, each of said pockets being capable of receiving only one of said particles;
B) placing a sample containing said particles dispersed in a liquid into said processing chamber;
C) causing at least some of the particles in said placed sample to be received by said particle receptor so that said received particles are all in the same plane;
D) processing at least some of said particles received by said particle receptor; and,
E) discarding said processing chamber containing said particles after step D).