Universal supports for oligonucleotide synthesis
View Patent ↗Universal supports for oligonucleotide synthesis include a support material represented by the following formula: In this formula, substituent A is selected from H, alkyl, aryl, or a polymeric or silica base material; substituent B is selected from acyl, aroyl, or a polymeric or silica base material; and substituent C is selected from a dimethoxytrityl group or a protecting group removable under acidic or neutral conditions. For the supports, one of substituents A or B constitutes the polymeric or silica base material. In use, an oligonucleotide is attached to the support at substituent C.
1. A support for oligonucleotide synthesis comprising: a support material represented by the following formula:
wherein substituent A is a member selected from the group consisting of a polymeric base material and a silica base material;
wherein substituent B is a member selected from the group consisting of an acyl group and an aroyl group; and
wherein substituent C is a member selected from the group consisting of a dimethoxytrityl group and a protecting group removable under acidic or neutral conditions.
2. A support according to claim 1 , wherein substituent A constitutes a long chain alkylamino controlled pore glass base material.
3. A support according to claim 2 , wherein sub stituent A includes the following substituent:
wherein lcaaCPG and its accompanying substituent groups represent the long chain alkylamino controlled pore glass base material.
4. A support according to claim 2 , wherein substituent B is an acyl group.
5. A support according to claim 2 , wherein substituent B is a dichioroacetyl group.
6. A support according to claim 2 , wherein substituent B is a formyl group.
7. A support according to claim 2 , wherein substituent B is a chioroacetyl group.
8. A support according to claim 2 , wherein substituent B is a trichioroacetyl group.
9. A support according to claim 2 , wherein substituent B is a 4-chlorophenoxyacetyl group.
10. A support according to claim 2 , wherein substituent B is a 2,4-dichiorophenoxyacetyl group.
11. An article represented by the following formula:
wherein substituent A is a member selected from the group consisting of a polymeric base material, and a silica base material;
wherein substituent B is a member selected from the group consisting of an acyl group and an aroyl group; and
wherein substituent C is a member selected from the group consisting of a dimethoxytrityl group, a nucleotide-containing group, and a protecting group removable under acidic or neutral conditions.
12. An article according to claim 11 , wherein substituent A constitutes a long chain alkylamino controlled pore glass base material.
13. An article according to claim 12 , wherein substituent A includes the following substituent:
wherein lcaaCPG and its accompanying substituent groups represent the long chain alkylamino controlled pore glass base material.
14. An article according to claim 12 , wherein sub stituent A includes the following substituent:
wherein Oligoprep 250 and its accompanying substituent groups represent the PVA base material.
15. An article according to claim 12 , wherein substituent B is an acyl group.
16. An article according to claim 12 , wherein substituent B is a dichioroacetyl group.
17. An article according to claim 12 , wherein substituent B is a formyl group.
18. An article according to claim 12 , wherein substituent B is a chloroacetyl group.
19. An article according to claim 12 , wherein substituent B is a trichioroacetyl group.
20. An article according to claim 12 , wherein substituent B is a 4-chiorophenoxyacetyl group.
21. An article according to claim 12 , wherein substituent B is a 2,4-dichlorophenoxyacetyl group.
22. An article according to claim 11 , represented by the following formula:
wherein lcaaCPG and its accompanying substituent groups represent a long chain alkylamino controlled pore glass base material.
23. An article according to claim 11 , represented by the following formula:
wherein lcaaCPG and its accompanying substituent groups represent a long chain alkylamino controlled pore glass base material.
24. An article according to claim 11 , wherein substituent C is a nucleotide-containing group including at least one nucleotide selected from the group consisting of: thymidine, 2′-deoxyadenosine, 2′-deoxycytindine, and 2′-deoxyguanosine.
25. An article according to claim 24 , wherein the nucleotide-containing group is attached via a phosphotriester group.
26. An article according to claim 11 , wherein substituent C is a nucleotide-containing group including a plurality of nucleotides.
27. An article according to claim 11 , wherein substituent C is a nucleotide-containing group.