Flea ultraspiracle nucleic acid molecules and uses thereof
View Patent ↗We claim a flea ultraspiracle nucleic acid molecule encoding an ultraspiracle protein, and a composition comprising the isolated nucleic acid molecule. Also, we claim a method of recombinantly producing the ultraspiracle protein thereof.
1. An isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of (a) a nucleic acid sequence that encodes a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:68, and SEQ ID NO:69, wherein said protein has ultraspiracle activity; and (b) a nucleic acid sequence fully complementary to the nucleic acid sequence of (a).
2. The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:31.
3. The nucleic acid molecule of claim 1 , wherein said nucleic acid molecule encodes a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:68, and SEQ ID NO:69.
4. A recombinant molecule comprising a nucleic acid molecule as set forth in claim 1 (a) operatively linked to a transcription control sequence.
5. A recombinant cell comprising a nucleic acid molecule as set forth in claim 1 (a).
6. A composition comprising an excipient and an isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of (a) a nucleic acid sequence that encodes a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:27 SEQ ID NO:68, and SEQ ID NO:69, wherein said protein has ultraspiracle activity; and (b) a nucleic acid sequence fully complementary to the nucleic acid sequence of (a).
7. The composition of claim 6 , wherein said nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:31.
8. The composition of claim 6 , wherein said nucleic acid molecule encodes a protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:68, and SEQ ID NO:69.
9. A method to produce a protein, said method comprising (a) culturing a cell transformed with an isolated nucleic acid molecule comprising a nucleic acid sequence that encodes a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:68, and SEQ ID NO:69, wherein said protein has ultraspiracle activity; and (b) recovering the expressed protein.
10. A composition comprising an isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of (a) a nucleic acid sequence that encodes a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:27, SEQ ID NO:68, SEQ ID NO:69, wherein said protein has ultraspiracle activity; and (b) a nucleic acid sequence fully complementary to a nucleic acid sequence of (a).