Ovomucoid promoters and methods of use
View Patent ↗The present invention includes nucleic acid molecules comprising an artificial chromosome and an avian ovomucoid gene expression controlling region operably linked to the coding sequence of a useful polypeptide.
1. A nucleic acid molecule comprising an ovomucoid gene expression controlling region isolated from a chicken which comprises a nucleotide sequence 95% homologous to nucleotides 1 to 68,295 of SEQ ID NO: 36.
2. The nucleic acid molecule of claim 1 wherein the nucleic acid is DNA.
3. The nucleic acid molecule of claim 1 comprising an attB site.
4. The nucleic acid molecule of claim 1 comprising a signal sequence coding region.
5. The nucleic acid of claim 1 comprising an artificial chromosome.
6. The nucleic acid of claim 1 comprising an IRES.
7. The nucleic acid molecule of claim 1 comprising an artificial chromosome selected from the group consisting of a BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), HAC (human artificial chromosome), MAC (mammalian artificial chromosome), BBPAC (bacteriophage derived artificial chromosome) and PAC (P1 derived artificial chromosome).
8. The nucleic acid of claim 1 wherein the ovomucoid gene expression controlling region comprises a sequence at least 99% homologous to nucleotides 1 to 68,295 of SEQ ID NO: 36.
9. The nucleic acid of claim 1 wherein the ovomucoid gene expression controlling region comprises a the sequence of nucleotides 1 to 68,295 of SEQ ID NO: 36.
10. The nucleic acid molecule of claim 1 comprising a nucleotide sequence encoding a polypeptide other than ovomucoid protein operably linked to the ovomucoid gene expression controlling region.
11. The nucleic acid molecule of claim 1 wherein a nucleotide sequence encoding a protein of pharmaceutical interest is operably linked to the ovomucoid gene expression controlling region.
12. The nucleic acid molecule of claim 1 wherein a nucleotide sequence encoding a light chain or a heavy chain of an antibody is operably linked to the ovomucoid gene expression controlling region.
13. The nucleic acid molecule of claim 12 wherein the antibody is selected from the group consisting of IgG, IgA, IgD, IgM and IgE.
14. The nucleic acid molecule of claim 12 wherein the antibody is IgG.
15. The nucleic acid molecule of claim 12 wherein the antibody is IgG1.
16. The nucleic acid molecule of claim 1 wherein a nucleotide sequence encoding a portion of light chain or portion of a heavy chain of an antibody is operably linked to the ovomucoid gene expression controlling region.
17. The nucleic acid molecule of claim 16 wherein the antibody is selected from the group consisting of IgG, IgA, IgD, IgM and IgE.
18. The nucleic acid molecule of claim 16 wherein the antibody is IgG.
19. The nucleic acid molecule of claim 16 wherein the antibody is IgG1.
20. The nucleic acid molecule of claim 1 wherein a nucleotide sequence encoding a hormone is operably linked to the ovomucoid gene expression controlling region.
21. A nucleic acid molecule comprising an ovomucoid gene expression controlling region isolated from a chicken which comprises a nucleotide sequence 95% homologous to nucleotides 1 to 68,295 of SEQ ID NO: 36 and a coding sequence encoding a polypeptide other than ovomucoid wherein the polypeptide other than ovomucoid protein is operably linked to the ovomucoid gene expression controlling region.
22. The nucleic acid molecule claim 21 wherein the nucleic acid is DNA.
23. The nucleic acid molecule of claim 21 comprising an attB site.
24. The nucleic acid molecule of claim 21 comprising a signal sequence coding region.
25. The nucleic acid molecule of claim 21 comprising an IRES.
26. The nucleic acid of claim 21 comprising an artificial chromosome.
27. The nucleic acid molecule of claim 21 comprising an artificial chromosome selected from the group consisting of a BAG (bacterial artificial chromosome), YAC (yeast artificial chromosome), HAC (human artificial chromosome), MAC (mammalian artificial chromosome), BBPAC (bacteriophage derived artificial chromosome) and PAC (P1 derived artificial chromosome).
28. The nucleic acid molecule of claim 21 wherein the ovomucoid gene expression controlling region comprises a sequence at least 99% homologous to nucleotides 1 to 68,295 of SEQ ID NO: 36.
29. The nucleic acid of claim 21 wherein the ovomucoid gene expression controlling region comprises a the sequence of nucleotides 1 to 68,295 of SEQ ID NO: 36.
30. A cell in culture containing the nucleic acid molecule of claim 21 .
31. The nucleic acid molecule of claim 21 wherein the polypeptide is a fusion protein.
32. The nucleic acid molecule of claim 21 wherein the polypeptide is a CTLA4-Fc fusion protein.
33. The nucleic acid molecule of claim 21 wherein a nucleotide sequence encoding a protein of pharmaceutical interest is operably linked to the ovomucoid gene expression controlling region.
34. The nucleic acid molecule of claim 21 wherein a nucleotide sequence encoding a light chain or a heavy chain of an antibody is operably linked to the ovomucoid gene expression controlling region.
35. The nucleic acid molecule of claim 34 wherein the antibody is selected from the group consisting of IgG, IgA, IgD, IgM and IgE.
36. The nucleic acid molecule of claim 34 wherein the antibody is IgG.
37. The nucleic acid molecule of claim 34 wherein the antibody is IgG1.
38. The nucleic acid molecule of claim 21 wherein a nucleotide sequence encoding a portion of light chain or portion of a heavy chain of an antibody is operably linked to the ovomucoid gene expression controlling region.
39. The nucleic acid molecule of claim 38 wherein the antibody is selected from the group consisting of IgG, IgA, IgD, IgM and IgB.
40. The nucleic acid molecule of claim 38 wherein the antibody is IgG.
41. The nucleic acid molecule of claim 38 wherein the antibody is IgG1.
42. The nucleic acid molecule of claim 21 wherein a nucleotide sequence encoding a hormone is operably linked to the ovomucoid gene expression controlling region.