Composition, methods and reagents for the synthesis of a soluble form of human PHEX
View Patent ↗This invention relates to a soluble form of PHEX, PHEX being a type II integral membrane glycoprotein. This enzyme is the gene product of a phosphate-regulating gene with homologies to endopeptidases on the X chromosome. To produce a soluble form of PHEX, the transmembrane anchor domain has been modified to encode a signal peptidase coding sequence. The soluble PHEX therefore comprises the active ectodomain. An inactive mutant of PHEX is also an object of this invention. Both soluble and inactive mutant forms of PHEX can be used to screen ligands to PHEX. These ligands can also be used as substrates or inhibitors of PHEX. PHEX being phosphaturic, an inhibitor thereof will be used to treat phosphaturia and/or hypophosphatemia. On the opposite, a substrate for PHEX or PHEX itself can be used to treat hyperphosphatemia.
1. A variant of the polypeptide of SEQ ID NO: 1, wherein the variant differs solely from SEQ ID NO: 1 by (1) a replacement of amino acid residues 28 to 36 of SEQ ID NO: 1 by a more hydrophilic domain; and (2) removal of residues 37 to 40 of SEQ ID NO: 1.
2. The variant of claim 1 , wherein the more hydrophilic domain consists of amino acids 2 to 10 of SEQ ID NO: 4.
3. An antigenic composition, which comprises the variant of claim 1 .
4. An antigenic composition, which comprises the variant of claim 2 .
5. A composition comprising the variant of claim 1 and a pharmaceutically acceptable carrier.
6. A composition comprising the variant of claim 2 and a pharmaceutically acceptable carrier.
7. A device for screening Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome (PHEX) ligands, which comprises a soluble PHEX enzyme fixed onto a solid support, wherein said enzyme is the variant of claim 1 .
8. A device for screening Phosphate Regulating Gene With Homologies To Endopeptidases On The X Chromosome (PHEX) ligands, which comprises a soluble PHEX enzyme fixed onto a solid support, wherein said enzyme is the variant of claim 2 .
9. The device of claim 8 , wherein said variant is fixed onto the solid support through its binding to an anti-PHEX antibody itself fixed onto said solid support.
10. The device of claim 8 , wherein said variant is fixed onto the solid support through a C-terminal amino acid extension ending with a residue or group capable of coupling the variant to the solid support.
11. The device of claim 7 , wherein said variant is fixed onto the solid support through its binding to an anti-PHEX antibody itself fixed onto said solid support.
12. The device of claim 7 , wherein said variant is fixed onto the solid support through a C-terminal amino acid extension ending with a residue or group capable of coupling the variant to the solid support.
13. A soluble PHEX enzyme, wherein said enzyme is a variant of the polypeptide of SEQ ID NO: 1, and wherein said variant is obtained by the process of (1) modifying a nucleic acid encoding the polypeptide of SEQ ID NO: 1, wherein said modification results in (a) amino acid residues 28 to 36 of SEQ ID NO: 1 being replaced by a more hydrophilic domain, and (b) amino acid residues 37 to 40 of SEQ ID NO: 1 being deleted; (2) expressing the nucleic acid of (1) in a eukaryotic host cell; and (3) recovering the variant as a secretion product of said host cell.