System and method employing photokinetic techniques in cell biology imaging applications
View Patent ↗A system and method employing photokinetic techniques in cell biology imaging applications are disclosed. Systems and methods of acquiring image data of an object may comprise: selectively inducing photoactivation of material at a site on the object; performing an optical axis integration scan; simultaneously executing a time delay integration scan sequence; and processing acquired image data in accordance with one or more desired analyses. Various methodologies and applications may include, inter alia, selective photobleaching of a site on the object, diffusion rate, velocity, and wave-front propagation analyses, multi-dimensional analyses of dispersion characteristics, biomolecular binding in cellular organelles, and photoactivation assisted systematic image segmentation for the study of cellular components.
1. A method of acquiring image data of an object; said method comprising:
selectively inducing photoactivation of material at a site on the object;
performing an optical axis integration scan;
simultaneously executing a time delay integration scan sequence; and
selectively repeating said performing and said executing.
2. The method of claim 1 wherein said performing comprises acquiring image data of the object at an image plane positioned along an optical axis.
3. The method of claim 2 wherein said performing further comprises providing relative translation along said optical axis of the object and said image plane.
4. The method of claim 3 wherein said executing comprises providing relative translation along a lateral axis of the object and said image plane.
5. The method of claim 4 wherein said executing comprises synchronizing said relative translation along said lateral axis with a data acquisition rate associated with an imaging device.
6. The method of claim 3 wherein said performing further comprises selectively alternating a direction of said relative translation along said optical axis.
7. The method of claim 2 wherein said performing further comprises integrating said image data concomitantly with said acquiring.
8. The method of claim 7 further comprising deblurring said image data subsequent to said integrating.
9. The method of claim 7 further comprising deconvolving said image data subsequent to said integrating.
10. The method of claim 1 wherein said selectively inducing comprises tagging the object to be scanned with a Green Fluorescent Protein variant.