Alkaline protease from
View Patent ↗The invention relates to a novel alkaline protease of the subtilisin type from Bacillus sp. (DSM 14392) and various related proteins and derivatives thereof. The invention further relates to washing and cleaning agents comprising novel alkaline proteases of the subtilisin type, various related proteins and derivatives thereof and corresponding washing and cleaning methods and use thereof in washing and cleaning agents and further technical applications.
1. An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.
2. An isolated alkaline protease comprising the amino acid sequence of position 106 to position 374 of SEQ ID NO:2.
3. The isolated alkaline protease of claim 2 , wherein the alkaline protease is isolated from a microorganism.
4. The alkaline protease of claim 3 , wherein the microorganism is a gram-positive bacterium.
5. The alkaline protease of claim 4 , wherein the bacterium is of the genus Bacillus.
6. The alkaline protease of claim 4 , wherein the bacterium is the Bacillus sp deposited as accession number DSM 14392.
7. An isolated host cell that expresses the polypeptide of claim 1 .
8. The isolated host cell of claim 7 , wherein the cell is a bacterium.
9. The isolated host cell of claim 7 , wherein the cell secretes the polypeptide.
10. The isolated host cell of claim 7 , wherein the cell is a eukaryotic cell.
11. The isolated host cell of claim 10 , wherein the cell modifies the polypeptide posttranslationally.
12. A washing or cleaning product comprising the alkaline protease of claim 2 .
13. The product of claim 12 further comprising at least one of an additional protease, an amylase, a cellulase, a hemicellulase, an oxidoreductase, or a lipase.
14. A cosmetic comprising the alkaline protease of claim 2 and a suitable carrier.
15. An isolated alkaline protease comprising the amino acid sequence of SEQ ID NO:3.
16. An isolated alkaline protease encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 1.