Diffusion promoting device for low flow velocity gradient high performance liquid chromatography
Device for improving detection sensitivity in low flow velocity high performance liquid chromatographic apparatus. Preferredly, diffusion promoting device connected at just before separation column and having function of improving detection sensitivity for use in low flow velocity high performance liquid chromatographic apparatus. Also, method for improving detection sensitivity in low flow velocity high performance liquid chromatographic apparatus.
1. A high performance liquid chromatographic apparatus size-dimensioned to have a flow velocity gradient of 250 microliters per minute or less, said apparatus in which a solvent pump (P 1 ), an injector (I), and a switching valve (V) are connected in this order in one line; and a solvent pump (P 2 ), a switching valve (V), a diffusion promoting device (DU) that comprises a solvent inlet tube and a solvent outlet tube, wherein said device includes at least one feature selected from the group consisting of (ii) having a solvent outlet tube connected to a solvent inlet tube at an acute angle, a right angle, or an obtuse angle and (iii) having a solvent outlet tube connected to a solvent inlet tube by a connecting part having a diameter that is larger than the diameters of the diameters of the solvent inlet and outlet tubes, a separation column (C), and a detector (D) are connected in another line.
2. A high performance liquid chromatographic apparatus apparatus size-dimentioned to have a flow velocity gradient of 250 microliters per minute or less, said apparatus in which a solvent pump (P 1 ), a switching valve (V), a solvent mixer (MC), and a switching valve (V) are connected in this order in one line; a solvent pump (P 2 ), a switching valve (V), a diffusion promoting device (DU) that comprises a solvent inlet tube and a solvent outlet tube, wherein said device includes at least one feature selected from the group consisting of (i) having a solvent inlet tube and a solvent outlet tube with different inner diameters, (ii) having a solvent outlet tube connected to a solvent inlet tube at an acute angle, a right angle, or an obtuse angle, and (iii) having a solvent outlet tube connected to a solvent inlet tube by a connecting part having a diameter that is larger than the diameters of the diameters of the solvent inlet and outlet tubes, a separation column (C), and a detector (D) are connected in another line; and a switching valve (V), a component concentration column (M), and a switching valve (V) are connected in a different line.
3. The high performance liquid chromatographic apparatus of claim 2 , wherein the diffusion promoting device (i) has a solvent inlet tube and a solvent outlet tube with different inner diameters.
4. The high performance liquid chromatographic apparatus of claim 2 , wherein the diffusion promoting device (ii) has a solvent outlet tube connected to a solvent inlet tube at an acute angle, a right angle, or an obtuse angle.
5. The high performance liquid chromatographic apparatus of claim 4 , wherein the diffusion promoting device (ii) has a solvent outlet tube connected to a solvent inlet tube at a right angle.
6. The high performance liquid chromatographic apparatus of claim 2 , wherein the diffusion promoting device (iii) has a solvent outlet tube connected to a solvent inlet tube by a connecting part having a diameter that is larger than the diameters of the diameters of the solvent inlet and outlet tubes.