Postpartum-derived cells for use in treatment of disease of the heart and circulatory system
View Patent ↗Cells derived from postpartum tissue and methods for their use in treatment of diseases of the heart or circulatory system are disclosed. Cells may be used in either differentiated or undifferentiated forms, in homogenous cultures, or as populations with other cells, and in conjunction with other bioactive factors.
1. A method for maintaining cardiac function as measured by fractional shortening following myocardial infarction in a patient, the method comprising
administering by injection to the ischemic myocardium of the patient a homogeneous population of cells in an amount effective to maintain cardiac function as measured by fractional shortening following myocardial infarction,
wherein the homogeneous population of cells is isolated from human umbilical cord tissue substantially free of blood, does not express CD31, CD34, CD45, CD80, CD86, CD178, B7-H2, HLA-G, CD117, CD141 and HLA-DR, DP, DQ, does express CD10, CD13, CD44, CD73, CD90 PDGFr-alpha PD-L2 and HLA-A, B, C, has the potential for at least 40 doublings in culture, maintains a normal karyotype upon passaging, has the potential to express GATA4, and has increased expression of an endogenous gene encoding interleukin 8 and an endogenous gene encoding reticulon 1 relative to expression of an endogenous gene encoding interleukin 8 and an endogenous gene encoding reticulon 1 by a human cell that is a fibroblast, a mesenchymal stem cell, or an iliac crest bone marrow cell.
2. The method of claim 1 , wherein the homogenous cell population is administered with at least one other agent.
3. The method of claim 2 , wherein the at least one other agent is an antithrombogenic agent, an anti-inflammatory agent, an immunosuppressive agent, an immunomodulatory agent, or an antiapoptotic agent.
4. The method of claim 2 , wherein the at least one other agent is administered simultaneously with, or before, or after, the cell population.
5. A method for maintaining cardiac function as measured by fractional shortening following myocardial infarction in a patient, the method comprising administering by injection to the ischemic myocardium of the patient a substantially homogeneous population of cells in an amount effective to maintain cardiac function as measured by fractional shortening following myocardial infarction, wherein the cells are isolated from human umbilical cord tissue substantially free of blood and identified by ATCC Accession No. PTA-6067.
6. A method for maintaining cardiac function as measured by fractional shortening following myocardial infarction in a patient, the method comprising administering by injection to the ischemic myocardium of the patient a substantially homogeneous population of cells in an amount effective to maintain cardiac function as measured by fractional shortening following myocardial infarction, wherein the cells are isolated from human umbilical cord tissue substantially free of blood and identified by ATCC Accession No. PTA-6068.