Elevation of oil levels in plants
View Patent ↗This present invention provides a method for increasing oil levels in corn kernel tissue by expression of an HOI001 GBSS allele. The present invention also provides isolated nucleic acid molecules encoding a HOI001 GBSS polypeptide.
1. A substantially purified nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising SEQ ID NO: 1 or the complement thereof;
b) a nucleic acid molecule comprising SEQ ID NO; 11 or the complement thereof; and
c) a nucleic acid molecule which encodes a polypeptide having at least 95% amino acid identity with SEQ ID NO: 3.
2. An expression cassette comprising a nucleic acid molecule of claim 1 , wherein said nucleic acid molecule is operably linked to a promoter, which is functional in a plant cell.
3. A plant cell comprising the expression cassette of claim 2 .
4. A plant comprising the plant cell of claim 3 .
5. A plant according to claim 4 , wherein the plant is a monocot.
6. A plant according to claim 5 , wherein the monocot plant is corn.
7. Seeds obtained from a corn plant, wherein the seeds comprise the nucleic acid molecule of claim 1 .
8. A plant having stably incorporated into its genome a nucleic acid molecule selected from the group consisting of:
a) a nucleic acid molecule comprising SEQ ID NO: 1 or the complement thereof;
b) a nucleic acid molecule comprising SEQ ID NO: 11 or the complement thereof; and
c) a nucleic acid molecule which encodes a polypeptide having at least 95% amino acid identity with SEQ ID NO: 3.
9. A transgenic plant according to claim 8 , wherein the plant is a monocot.
10. The monocot plant of claim 9 , wherein the plant is corn.
11. Transgenic seeds obtained from the plant of claim 10 , wherein the seeds comprise said nucleic acid molecule.
12. A method of producing a plant having increased levels of oil production, wherein the method comprises:
(a) transforming a plant with an expression cassette comprising a nucleic acid molecule selected from the group consisting of:
i) a nucleic acid molecule comprising SEQ ID NO: 1 or the complement thereof;
ii) a nucleic acid molecule comprising SEQ ID NO: 11 or the complement thereof; and
iii) a nucleic acid molecule which encodes a polypeptide having at least 95% amino acid identity with SEQ ID NO: 3; wherein said expression cassette further comprises a promoter region functional in a plant cell, operably linked to said nucleic acid molecule; and
(b) growing the transformed plant.
13. The method claim 12 , wherein the plant is a monocot.
14. The method of claim 13 , wherein the monocot plant is corn.
15. The method of claim 14 , wherein the promoter region is an endosperm-preferred promoter region.
16. The method of claim 15 , wherein the promoter region is the Z27 promoter region.