IP Library › Granted Patent US 7,144,719
Granted Patent B2
US 7,144,719 · App. 10/888,588 · Granted Dec 5, 2006

Method for producing protease

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Quick Facts
Patent No.
US 7,144,719
App. No.
10/888,588
Granted
Dec 5, 2006
Kind
B2
Abstract

A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.

Claims (13)

1. An isolated polynucleotide encoding an amino acid sequence according to formula I:

SIG-(SEQ ID NO:30)-PRO [I]

wherein SIG represents the amino acid sequence SEQ ID NO:3 of a signal peptide derived from a subtilisin, PRO represents the amino acid sequence of a protease consisting of an amino acid sequence in which one or more amino acid residues are deleted from the C-terminus of the amino acid sequence of SEQ ID NO:4 and having a protease activity, wherein said protease amino acid sequence comprises the amino acid sequence of SEQ ID NO:1.

2. The polynucleotide according to claim 1 , which is contained in the plasmid pSPO124ΔC.

3. A method of producing a protease of interest, comprising:

culturing a bacterium of genus Bacillus into which the polynucleotide according to claim 1 is introduced; and

collecting said protease from the culture.

4. The method of producing a protease according to claim 3 , wherein the bacterium of genus Bacillus is Bacillus subtilis.

5. The method of producing a protease according to claim 3 , wherein the polynucleotide is introduced into the bacterium of genus Bacillus by means of a plasmid vector.

6. The method of producing a protease according to claim 5 , wherein the plasmid pSPO124ΔC is introduced into the bacterium of genus Bacillus.

7. The method of producing a protease according to claim 5 , comprising culturing Bacillus subtilis DB104/pSPO124ΔC FERM BP-6294, and collecting the protein of interest from the culture.

8. A plasmid vector into which the polynucleotide according to claim 1 is inserted.

9. The plasmid vector according to claim 8 , which is pSPO124ΔC.