Device and method for culturing cells
View Patent ↗An object of the present invention is to construct a culture device optimized for culturing animal cells. The present invention provides a device for culturing cells which comprises at least one water-containing polymer gel film for adhering animal cells onto at least one surface of the film, and has a structure capable of supplying different liquids to both sides of the film.
1. A device for culturing cells which comprises:
at least one water-containing polymer gel film for adhering animal cells onto at least one surface of the film,
a structure capable of supplying different liquids to both sides of the film wherein the structure comprises at least one flow channel, formed by the water-containing gel film and an inner wall of a reactor, for supplying a culture solution to a cell culture section having a uniform width, and
a means for holding the water-containing polymer gel film between the flow channels,
wherein a pocket structure is provided for establishing a uniform dynamic pressure of the culture solution across the uniform width while culture solution is introduced through an inlet pipe, so as to establish a uniform flow rate by applying a flow shearing force to the animal cells.
2. The device according to claim 1 , wherein the at least one flow channels is provided on one and the other sides of the water-containing polymer gel film in such a way that the different liquids can flow to one and the other sides of the film.
3. The device according to claim 1 , which comprises at least one water-containing polymer gel film for adhering animal cells onto at least one surface of the film, at least one flow channels provided on one and the other sides of the water-containing polymer gel film in such a way that different liquids can flow to one and the other side of the film, and a means for holding the water-containing polymer gel film between the flow channels.
4. The device according to claim 1 , wherein one surface of the water-containing polymer gel film is covered with an animal cell adhesive material.
5. The device according to claim 1 , wherein the flow shearing force applied to the cells is at least 2.0 dyn/cm 2 .
6. The device according to claim 1 , wherein the at least one flow channels is formed of the water-containing polymer gel film and an inner wall of the reactor, and the spacing between the water-containing polymer gel film and the inner wall of the reactor falls within 10 μm to 2 mm, both inclusive.
7. The device according to claim 1 , wherein 90% to 100% region, both inclusive, of the surface of the water-containing polymer gel film is coated with the animal cells which are adhered to the film.
8. The device according to claim 1 , wherein the water-containing polymer gel film contains chitosan.
9. The device according to claim 1 , wherein the water-containing polymer gel film has a dry film thickness of 5 μm to 200 μm, both inclusive.
10. The device according to claim 1 , wherein 2 to 10 types of animal cells are used as the animal cells.
11. The device according to claim 1 , wherein the animal cells are stacked in 2 to 10 layers.
12. A method of culturing animal cells, which comprises supplying different liquids to one and the other sides of a water-containing polymer gel film having animal cells adhered onto at least one of the surfaces thereof by using the device according to claim 1 .