Canine specific growth hormone releasing hormone
View Patent ↗A composition and a method of increasing growth hormone (“GH”) values in a canine or dog, and more specifically, a canine- or dog-specific growth hormone releasing hormone (“dGHRH”), or functional biological equivalent thereof. The dGHRH is an isolated composition or a nucleic acid molecule that encodes the dGHRH or functional biological equivalent. Also, a method for delivering the composition of this invention to a subject, wherein the dGHRH increases the level of growth hormone (“GH”) secretion in a recipient subject, such as a canine or dog.
1. A composition comprising a nucleic acid expression construct that encodes the canine specific growth hormone releasing hormone (“dGHRH”) consisting of SEQ ID NO.: 04.
2. The composition of claim 1 , wherein the dGHRH increases growth hormone (“GH”) in a recipient subject.
3. The composition of claim 1 , wherein the dGHRH improves hematological parameters in a recipient subject.
4. The composition of claim 1 , wherein the dGHRH increases red blood cell values in a recipient subject.
5. The composition of claim 1 , wherein the dGHRH increases hemoglobin values in a recipient subject.
6. The composition of claim 1 , wherein the dGHRH increases mean corpuscular hemoglobin values in a recipient subject.
7. The composition of claim 1 , wherein the nucleic acid expression construct further comprises:
I. a synthetic or eukaryotic promoter;
II. a poly-adenylation signal;
III. a selectable marker gene promoter;
IV. a ribosomal binding site;
V. a selectable marker gene sequence; and
VI. an origin of replication.
8. The composition of claim 7 , wherein the polyadenylation signal comprises a portion of the human growth hormone 3′-untranslated region.
9. A composition comprising a nucleic acid expression construct that encodes the canine specific growth hormone releasing hormone consisting of SEQ ID NO.:03.