Polynucleotides encoding SREB1 receptor
This invention belongs to the genetic engineering field, and provides novel G protein-coupled receptor family proteins SREB1, SREB2 and SREB3 expressed in the central nervous system, genes coding for these proteins, screening methods using these proteins and so on. As one of the methods for obtaining the G protein-coupled receptor proteins of the present invention, RT-PCR is carried out using mRNA extracted from human or rat brain tissue or brain-derived cells as the template and using two primers interposing the entire portion or a part of the G protein-coupled receptor protein translation region, thereby obtaining cDNA corresponding to the G protein-coupled receptor protein or a part thereof, and the cDNA is integrated into an appropriate expression vector and expressed in a host cell.
1. An isolated polynucleotide encoding a G protein-coupled receptor protein comprising amino acids 1 to 375 of SEQ ID NO:2.
2. The isolated polynucleotide encoding a G protein-coupled receptor protein of claim 1 , which encodes a polypeptide consisting of amino acids 1 to 375 of SEQ ID NO:2.
3. An expression vector comprising the polynucleotide of claim 1 or 2 .
4. A host cell transformed or transfected with the expression vector of claim 3 .
5. A process for producing a recombinant host cell comprising transforming or transfecting a cell with the expression vector of claim 3 such that the host cell, under appropriate conditions, produces said G protein-coupled receptor protein.
6. A membrane of the recombinant host cell of claim 4 expressing said polypeptide.
7. A method for producing a G protein-coupled receptor protein comprising culturing the host cell of claim 4 under conditions sufficient for the production of said G protein-coupled receptor protein and recovering said protein from the culture.
8. A method for producing a G protein-coupled receptor protein which has the amino acid sequence comprising amino acids 1 to 375 of SEQ ID NO:2 comprising culturing the host cell of claim 4 .