Confocal microscope
View Patent ↗A confocal microscope comprises a light source unit having at least two light sources which generate lights having different wavelengths, an objective lens which condenses light from the light source unit on a sample, a light scanning unit which scans the light form the light source unit on the sample two-dimensionally, and a photonic crystal fiber which is disposed between the light source unit and the light scanning unit, and which propagates the light led from the light source unit to the light scanning unit side, wherein the photonic crystal fiber has a plurality of air holes arranged at a clad provided at a periphery of a core.
1. A confocal microscope comprising:
a light source unit including at least two light sources which generate lights having different wavelengths;
an objective lens which condenses light from the light source unit on a sample;
a light scanning unit which two-dimensionally scans the light from the light source unit on the sample; and
a photonic crystal fiber which is disposed between the light source unit and the light scanning unit, and which propagates the light from the light source unit to the light scanning unit side,
wherein the photonic crystal fiber comprises a core and a clad provided at a periphery of the core, and a plurality of air holes provided in the clad; and
wherein an arrangement of the air holes differs in a vertical direction and in a horizontal direction in the photonic crystal fiber to propagate the light from the light source unit while maintaining a polarization characteristic of the light.
2. The confocal microscope according to claim 1 , wherein said plurality of air holes include air holes having a first diameter and air holes having a second diameter different from the first diameter.
3. The confocal microscope according to claim 2 , wherein a wavelength characteristic of an optical system to which light is led from the photonic crystal fiber is optically compensated with respect to a wavelength characteristic of an NA at the photonic crystal fiber.
4. The confocal microscope according to claim 2 , wherein a wavelength characteristic of an optical system to which light is led from the photonic crystal fiber side is optically compensated such that a spot diameter formed by the objective lens is constant.
5. The confocal microscope according to claim 1 , wherein at least one of the at least two light sources of the light source unit comprises a light emitting diode.
6. The confocal microscope according to claim 1 , wherein the at least two light sources of the light source unit comprise light emitting diodes.
7. The confocal microscope according to claim 1 , wherein a wavelength characteristic of an optical system to which light is led from the photonic crystal fiber is optically compensated with respect to a wavelength characteristic of an NA at the photonic crystal fiber.
8. The confocal microscope according to claim 1 , further comprising a collimator lens which collimates an outgoing beam from the photonic crystal fiber, and
wherein a wavelength dependency of NA of the photonic crystal fiber is compensated by varying NA of the collimator lens.
9. The confocal microscope according to claim 1 , wherein said plurality of air holes are not formed within a region at a predetermined distance from an end face of the photonic crystal fiber.
10. The confocal microscope according to claim 9 , wherein the predetermined distance is from 50 to 500 μm.
11. The confocal microscope according to claim 9 , wherein said plurality of air holes include air holes having a first diameter and air holes having a second diameter different from the first diameter.
12. The confocal microscope according to claim 11 , wherein a wavelength characteristic of an optical system to which light is led from the photonic crystal fiber is optically compensated with respect to a wavelength characteristic of an NA at the photonic crystal fiber.
13. The confocal microscope according to claim 9 , wherein a wavelength characteristic of an optical system to which light is led from the photonic crystal fiber is optically compensated with respect to a wavelength characteristic of an NA at the photonic crystal fiber.
14. The confocal microscope according to claim 9 , wherein a wavelength characteristic of an optical system to which light is led from the photonic crystal fiber is optically compensated such that a spot diameter formed by the objective lens is constant.
15. The confocal microscope according to claim 9 , further comprising a collimator lens which collimates an outgoing beam from the photonic crystal fiber, and
wherein a wavelength dependency of NA of the photonic crystal fiber is compensated by varying NA of the collimator lens.
16. The confocal microscope according to claim 1 , wherein the light source unit emits light of visible range and at least one of violet, ultra-violet, near-infrared, and infrared ranges; and
wherein the photonic crystal fiber transmits light emitted from the light source unit to the light scanning unit via only one photonic crystal fiber.
17. The confocal microscope according to claim 1 , wherein the confocal microscope carries out a DIC observation using the light propagated by the photonic crystal fiber while the polarization characteristic of the light is maintained.