Ionic molecular conjugates of N-acylated derivatives of poly(2-amino-2-deoxy-D-glucose) and polypeptides
View Patent ↗A copolymer comprising an N-acylated derivative, and a composition comprising said copolymer and a polypeptide, said polypeptide comprising at least one effective ionogenic amine, wherein at least 50 percent, by weight, of said polypeptide present in said composition is ionically bound to said polymer.
1. A copolymer comprising an N-acylated derivative of poly(2-amino-2-deoxy-D-glucose), in which between 1 and 50 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with glutaryl and between 50 and 99 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a second acyl group, said second acyl group is COE 2 where E 2 is selected from the group consisting of C 1-30 alkyl, C 2-30 alkenyl, C 6-37 arylalkyl, and C 8-37 arylalkenyl, provided at least one of the free amines of said poly(2-amino-2-deoxy-D-glucose) is acylated with glutaryl.
2. A copolymer of claim 1 , wherein said second acyl group is propionyl.
3. A composition comprising a copolymer and a peptide, wherein said copolymer is an N-acylated derivative of poly(2-amino-2-deoxy-D-glucose) in which between 1 and 50 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a first acyl group, said first acyl group is COE 1 where E 1 is selected from the group consisting of C 3-33 carboxyalkyl, C 3-33 carboxyalkenyl, C 7-39 carboxyarylalkyl, and C 9-39 carboxyarylalkenyl, and between 50 and 99 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a second acyl group, said second acyl group is COE 2 where E 2 is selected from the group consisting of C 1-30 alkyl, C 2-30 alkenyl, C 6-37 arylalkyl, and C 8-37 arylalkenyl, provided at least one of the free amines of said poly(2-amino-2-deoxy-D-glucose) is acylated with said first acyl group and wherein said peptide is selected from the group consisting of
or a pharmaceutically acceptable salt thereof, where at least 50 percent, by weight, of said peptide or said pharmaceutically acceptable salt thereof present in said composition is ionically bound to said copolymer, wherein said first acyl group is succinyl and said second acyl group is acetyl.
4. A composition comprising a copolymer and a peptide, wherein said copolymer is an N-acylated derivative of poly(2-amino-2-deoxy-D-glucose) in which between 1 and 50 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a first acyl group, said first acyl group is COE 1 where E 1 is selected from the group consisting of C 3-33 carboxyalkyl, C 3-33 carboxyalkenyl, C 7-39 carboxyarylalkyl, and C 9-39 carboxyarylalkenyl, and between 50 and 99 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a second acyl group, said second acyl group is COE 2 where E 2 is selected from the group consisting of C 1-30 alkyl, C 2-30 alkenyl, C 6-37 arylalkyl, and C 8-37 arylalkenyl, provided at least one of the free amines of said poly(2-amino-2-deoxy-D-glucose) is acylated with said first acyl group and wherein said peptide is selected from the group consisting of:
(p-Glu-His-Trp-Ser-Tyr-D-Trp-Leu-Arg-Pro-Gly-NH 2 );
([D-Ser(t-Bu) 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Trp 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt;
([des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Ser(t-Bu) 6 , Azgly 10 ]-LHRH);
([D-His(Bzl) 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Leu 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Trp 6 , MeLeu 7 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt); and
([D-Nal 6 ]-LHRH; or
a pharmaceutically acceptable salt thereof, where at least 50 percent, by weight, of said peptide or said pharmaceutically acceptable salt thereof, present in said composition is ionically bound to said copolymer, wherein said first acyl group is succinyl and said second acyl group is acetyl.
5. A composition comprising said copolymer of claim 2 and a peptide selected from the group consisting of
or a pharmaceutically acceptable salt thereof, where at least 50 percent, by weight, of said peptide or said pharmaceutically acceptable salt thereof present in said composition is ionically bound to said copolymer.
6. A composition comprising said copolymer of claim 2 and a peptide selected from the group consisting of:
(p-Glu-His-Trp-Ser-Tyr-D-Trp-Leu-Arg-Pro-Gly-NH 2 );
([D-Ser(t-BU) 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Trp 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt;
([des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Ser(t-Bu) 6 , Azgly 10 ]-LHRH);
([D-His(Bzl) 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Leu 6 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt);
([D-Trp 6 , MeLeu 7 , des-Gly-NH 2 10 ]-LHRH(1-9)NHEt); and ([D-Nal 6 ]-LHRH; or
a pharmaceutically acceptable salt thereof, where at least 50 percent, by weight, of said peptide or said pharmaceutically acceptable salt thereof, present in said composition is ionically bound to said copolymer.
7. A composition comprising a copolymer of and parathyroid hormone, an analogue thereof or a pharmaceutically acceptable salt thereof, wherein said copolymer is an N-acylated derivative of poly(2-amino-2-deoxy-D-glucose) in which between 1 and 50 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a first acyl group, said first acyl group is COE 1 where E 1 is selected from the group consisting of C 3-33 carboxyalkyl, C 3-33 carboxyalkenyl, C 7-39 carboxyarylalkyl, and C 9-39 carboxyarylalkenyl, and between 50 and 99 percent of the free amines of said poly(2-amino-2-deoxy-D-glucose) are acylated with a second acyl group, said second acyl group is COE 2 where E 2 is selected from the group consisting of C 1-30 alkyl, C 2-30 alkenyl, C 6-37 arylalkyl, and C 8-37 arylalkenyl, provided at least one of the free amines of said poly(2-amino-2-deoxy-D-glucose) is acylated with said first acyl group and where at least 50 percent, by weight, of said parathyroid hormone, said analogue thereof or said pharmaceutically acceptable salt thereof, present in said composition is ionically bound to said copolymer, wherein said first acyl group is succinyl and said second acyl group is acetyl.
8. A composition comprising said copolymer of claim 2 and parathyroid hormone, an analogue thereof or a pharmaceutically acceptable salt thereof, where at least 50 percent, by weight, of said parathyroid hormone, said analogue thereof or said pharmaceutically acceptable salt thereof, present in said composition is ionically bound to said copolymer.