Composition of matter comprising library of analytes hybridized to nucleic acid array for generating complex formation and signaling domains
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
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41 . A composition of matter that comprises a library of analytes, said analytes being hybridized to an array of nucleic acids, said nucleic acids being fixed or immobilized to a solid support, wherein said hybridization between said analytes and said nucleic acids generate a domain for complex formation, and said composition further comprising a signaling entity complexed to said domain.
42 . The composition of claim 41 , wherein said library of analytes is derived from a biological source comprising organs, tissues or cells.
43 . The composition of claim 41 , wherein said analytes comprise genomic DNA, episomal DNA, unspliced RNA, mRNA, rRNA, snRNA or a combination of any of the foregoing.
44 . The composition of claim 41 , wherein said nucleic acid array comprises DNA, RNA or analogs thereof.
45 . The composition of claim 44 , wherein said analogs comprise PNA.
46 . The composition of claims 44 or 45 , wherein said nucleic acids or analogs are modified on any one of the sugar, phosphate or base moieties.
47 . The composition of claim 41 , wherein said solid support is porous or non-porous.
48 . The composition of claim 47 , wherein said porous solid support comprises polyacrylamide or agarose.
49 . The composition of claim 47 , wherein said non-porous solid support comprises glass or plastic.
50 . The composition of claim 41 , wherein said solid support is transparent, translucent, opaque or reflective.
51 . The composition of claim 41 , wherein said nucleic acids are directly or indirectly fixed or immobilized to said solid support.
52 . The composition of claim 41 , wherein said nucleic acids are indirectly fixed or immobilized to said solid support by means of a chemical linker or linkage arm.
53 . The composition of claim 41 , wherein said domain for complex formation comprises DNA-DNA hybrids, DNA-RNA hybrids, RNA-RNA hybrids, DNA-PNA hybrids or RNA-PNA hybrids.
54 . The composition of claim 41 , wherein said signaling entity complexed to said domain comprises proteins or intercalators.
55 . The composition of claim 54 , wherein said proteins comprise nucleic acid binding proteins which bind preferentially to double-stranded nucleic acid.
56 . The composition of claim 55 , wherein said nucleic acid binding proteins comprise antibodies.
57 . The composition of claim 56 , wherein said antibodies are specific for nucleic acid hybrids comprising DNA-DNA hybrids, DNA-RNA hybrids, RNA-RNA hybrids, DNA-PNA hybrids or RNA-PNA hybrids
58 . The composition of claim 54 , wherein said intercalators comprise ethidium bromide, diethidium bromide, acridine orange or SYBR Green.
59 . The composition of claim 41 , wherein said proteins generate a signal directly or indirectly.
60 . The composition of claim 59 , wherein said direct signal generation comprises a fluorescent compound, a phosphorescent compound, a chemiluminescent compound, a chelating compound, an electron dense compound, a magnetic compound, an intercalating compound, an energy transfer compound or a combination of any of the foregoing.
61 . The composition of claim 59 , wherein said indirect signal generation comprises an antibody, an antigen, a hapten, a receptor, a hormone, a ligand, an enzyme or a combination of any of the foregoing.
62 . The composition of claim 61 , wherein said enzyme catalyzes a reaction comprising a fluorogenic reaction, a chromogenic reaction or a chemiluminescent reaction.
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