Composition comprising an array which further comprises chimeric compositions
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
1. A composition of matter that comprises an array of solid surfaces comprising a plurality of discrete areas,
wherein the plurality of discrete areas comprises a first discrete area and a second discrete area, said first discrete area and said second discrete area each comprising:
a chimeric composition consisting of:
(i) a nucleic acid portion covalently attached to
(ii) a peptide portion, wherein
said nucleic acid portion of the chimeric composition of said first discrete area has a different sequence from that of said nucleic acid portion of the chimeric composition of said second discrete area,
said peptide portion of the chimeric composition of said first discrete area has a binding affinity for a first non-nucleic acid analyte of interest,
said peptide portion of the chimeric composition of said second discrete area has a binding affinity for a second non-nucleic acid analyte,
said first non-nucleic acid analyte is different from said second non-nucleic acid analyte, and
said nucleic acid portion of the chimeric composition consists essentially of DNA, a DNA analog, PNA, or combinations thereof,
wherein said covalently attached peptide portion of the chimeric composition of said first discrete area is different from said covalently attached peptide portion of the chimeric composition of said second discrete area.
2. The composition of claim 1 , wherein said nucleic acid portion is modified on a sugar, phosphate or base moiety.
3. The composition of claim 1 , wherein said solid surfaces are porous.
4. The composition of claim 3 , wherein said porous solid surfaces comprises polyacrylamide or agarose.
5. The composition of claim 1 , wherein said solid surfaces are nonporous.
6. The composition of claim 5 , wherein said non-porous solid surfaces comprise glass or plastic.
7. The composition of claim 1 , wherein said solid surfaces are transparent, translucent, opaque or reflective.
8. The composition of claim 1 , wherein said nucleic acid portions are directly or indirectly fixed or immobilized to said solid surfaces.
9. A composition of matter that comprises an array of solid surfaces comprising a plurality of discrete areas,
wherein the plurality of discrete areas comprises a first discrete area and a second discrete area, said first discrete area and said second discrete area each comprising:
a chimeric composition consisting of:
(i) a nucleic acid portion covalently attached to
(ii) a peptide portion, wherein
said nucleic acid portion of the chimeric composition of said first discrete area has a different sequence from that of said nucleic acid portion of the chimeric composition of said second discrete area,
said peptide portion of the chimeric composition of said first discrete area has a binding affinity for a first non-nucleic acid analyte of interest,
said peptide portion of the chimeric composition of said second discrete area has a binding affinity for a second non-nucleic acid analyte,
said first non-nucleic acid analyte is different from said second non-nucleic acid analyte,
said nucleic acid portion does not comprise sequences which are either identical or complementary to sequences that code for said peptide, and
said nucleic acid portion of the chimeric composition comprises DNA, DNA analogs, PNA, or combinations thereof,
wherein the nucleic acid portion of the chimeric composition of the first discrete area is hybridized to complementary sequences of nucleic acids fixed or immobilized to the first discrete area,
wherein the nucleic acid portion of the chimeric composition of the second discrete area is hybridized to complementary sequences of nucleic acids fixed or immobilized to the second discrete area, and
wherein said covalently attached peptide portion of the chimeric composition of said first discrete area is different from said covalently attached peptide portion of the chimeric composition of said second discrete area.
10. The composition of claim 9 , wherein said solid surfaces are porous.
11. The composition of claim 10 , wherein said porous solid surfaces comprise polyacrylamide or agarose.
12. The composition of claim 9 , wherein said solid surfaces are non-porous.
13. The composition of claim 12 , wherein said non-porous solid surfaces comprises glass or plastic.
14. The composition of claim 9 , wherein said solid surfaces are transparent, translucent, opaque or reflective.
15. The composition of claim 9 , wherein said nucleic acid portion is modified on a sugar, phosphate or base moiety.
16. The composition of claim 8 , wherein said nucleic acid portions are directly or indirectly fixed or immobilized to said solid surfaces through hybridization to complementary nucleic acid sequences.
17. The composition of claim 9 , wherein said nucleic acid portions are directly or indirectly fixed or immobilized to said solid surfaces through hybridization to complementary nucleic acid sequences.
18. The composition of claim 1 , wherein said peptide portion is not encoded by (a) sequences in said nucleic acid portion or (b) sequences complementary thereto.
19. The composition of claim 1 , wherein the nucleic acid portion of each chimeric composition is directly bound to the surface of the array.
20. The composition of claim 9 , wherein the nucleic acid portion of each chimeric composition is directly bound to the surface of the array.
21. The composition of claim 1 , wherein the nucleic acid portion of each chimeric composition is hybridized to a complementary nucleic acid that is immobilized on the solid surface.