Thermophilic hydrophobin proteins and applications for surface modification
View Patent ↗The present invention relates to a thermophilic hydrophobin, TT1, or a protein or polypeptide substantially similar thereto, derived from the thermophilic fungus Talaromyces thermophilus . The invention further relates to a polynucleotide encoding such hydrophobin, as well as to materials coated with such hydrophobin.
1. An isolated hydrophobin polypeptide, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, when compared to SEQ ID NO:1, is stable at 90° C., and wherein a surface coated with the polypeptide demonstrates a change in water contact angle of at least 20° as compared with the uncoated surface.
2. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide has at least 85% sequence identity, when compard to SEQ ID NO:1.
3. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide has at least 90% sequence identity, when compared to SEQ ID NO:1.
4. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide has at least 95% sequence identity, when compared to SEQ ID NO:1.
5. The polypeptide of claim 1 , wherein the amino acid sequence of the polypeptide comprises SEQ ID NO:1.
6. A composite structure comprising a polymer material having a thermophilic hydrophobin coating thereon wherein the wettability of the polymer is altered by the thermophilic hydrophobin coating as characterized by a difference in water contact angle of at least 20°, and wherein the thermophilie hydrophobin coating comprises a hydrophobin polypetide having at least 80% amino acid sequence identity when compared to SEQ ID NO.1.
7. A composite structure according to claim 6 wherein the polymer material is selected from the group consisting of a fluoropolymer, a polyolefin, ethylene copolymer, a polyamide, a polyimide, and a polyester.
8. A composite structure according to claim 7 wherein the fluoropolymer is selected from the group consisting of Teflon®, Tedlar® and Nafion®.
9. A composite structure according to claim 7 wherein the polyolefin is selected from the group consisting of Tyvek®, Bynel®, Polypropylene PP6D81 resin and low density polyethylene DPE1645.
10. A composite structure according to claim 7 wherein the ethylene copolymer is Surlyn®.
11. A composite structure according to claim 7 wherein the polyester is selected from the group consisting of Mylar®, and Sorona®.
12. A composite structure according to claim 7 wherein the polyamide is selected from the group consisting of Kevlar® and Nomex®.
13. A composite structure according to claim 7 wherein the polyimide is Kapton®.
14. A composite structure of claim 6 wherein the amino acid sequence of the polypeptide has at least 85% sequence identity, when compared to SEQ ID NO:1.
15. A composite structure of claim 6 wherein the amino acid sequence of the polypeptide has at least 90% sequence identity, when compared to SEQ ID NO:1.
16. A composite structure of claim 6 wherein the amino acid sequence of the polypeptide has at least 95% sequence identity, when compared SEQ ID NO:1.
17. A medical device comprising the composite of either of claim 6 .
18. An electronic device comprising the composite of either of claim 6 .