Rat calcium channel subunits and related probes, cell lines and methods
View Patent ↗Novel sequences for calcium channel α 2 δ-2 and α 2 δ-3 subunits are provided. Also provided are cell lines that express the novel calcium channel subunits of the invention. These cells may be used for identifying compounds capable of stimulating or inhibiting the activation of the calcium channels.
1. An isolated nucleic acid comprising a nucleotide sequence that encodes a calcium channel subunit having the amino acid sequence of SEQ ID NO: 3 or a fragment thereof which encodes a functional α 2 δ-2 subunit, wherein the fragment is a truncated form of SEQ ID NO: 3, wherein the truncation involves removing up to 20 amino acids from the α 2 and/or δ portions.
2. A recombinant nucleic acid molecule which comprises a nucleotide sequence as set forth in claim 1 , operably linked to control sequences to effect its expression.
3. The nucleic acid of claim 2 , wherein said control sequences are operable in vertebrate cells.
4. Recombinant host cells which contain the nucleic acid molecule of claim 2 .
5. Vertebrate host cells which contain the nucleic acid molecule of claim 3 .
6. The vertebrate host cells of claim 5 , wherein said cells further comprise an expression vector which comprises an expression control sequence operably linked to a nucleotide sequence which encodes an α 1 calcium channel subunit.
7. A method to prepare cells that display functional calcium ion channel, which method comprises culturing cells which display an α 1 subunit and which further comprise the nucleic acid molecule of claim 2 .
8. Recombinant cells prepared by the method of claim 7 .
9. The isolated nucleic acid of claim 1 wherein 10–15 of the up to 20 amino acids are removed.
10. The isolated nucleic acid of claim 1 which encodes SEQ ID NO:3.