Methods of genetic analysis of yeast
View Patent ↗Arrays of oligonucleotide probes which are complementary to a plurality of S. cerevisisae and S. pombe genes are disclosed. The arrays may be used to measure the expression levels of a plurality of genes simultaneously.
1. An array comprising a plurality of nucleic acid probes, wherein the plurality comprises each of the sequences listed in SEQ ID Nos. 1-120,855, and wherein each probe in the plurality of nucleic acid probes consists of one of the sequences listed in SEQ ID Nos. 1-120,855.
2. The array of claim 1 further comprising at least one probe that is the perfect complement of one of the sequences listed in SEQ ID Nos. 1-120,855.
3. The array of claim 1 wherein said plurality of nucleic acid probes is attached to a solid support.
4. The array of claim 1 wherein each probe is attached to the array in a different localized area that is determinable.
5. The array of claim 1 wherein the array comprises a plurality of beads wherein the probes are attached to the beads and the probes on a bead consist of one of the sequences listed in SEQ ID NO: 1-120,855.
6. The array of claim 1 wherein the array consists of a single contiguous solid support.
7. A method of monitoring gene expression in a biological sample comprising yeast nucleic acid, comprising:
isolating nucleic acid derived from the sample;
labeling the nucleic acid;
hybridizing the labeled nucleic acid to an array comprising a plurality of nucleic acid probes, wherein the plurality comprises each of the sequences listed in SEQ ID Nos. 1-120,855, and wherein each probe in the plurality of nucleic acid probes consists of one of the sequences listed in SEQ ID Nos. 1-120,855; and,
detecting a hybridization pattern, wherein the intensity of signal resulting from hybridization to probes on the array is used to monitor gene expression levels.
8. The method of claim 7 wherein said monitoring gene expression levels comprises comparing gene expression levels of nucleic acids derived from two or more different samples and further comprises the step of:
comparing said hybridization patterns between said nucleic acids derived from said two or more different samples.
9. The method of claim 7 wherein the labeled nucleic acid hybridized to the array consists essentially of DNA.
10. The method of claim 7 wherein the yeast is selected from the group consisting of Saccharomyces cerevisisae and Schizosaccharomyces pombe.
11. The method of claim 7 wherein the labeled nucleic acid hybridized to the array consists essentially of RNA that is in the sense orientation relative to the target mRNA.
12. The method of claim 7 wherein the labeled nucleic acid is hybridized to the array in a single reaction.
13. A method of monitoring gene expression in a yeast sample, comprising:
isolating nucleic acid derived from the yeast sample;
labeling the nucleic acid with a detectable label;
hybridizing the labeled nucleic acid to an array comprising a plurality of nucleic acid probes, wherein the plurality comprises each of the sequences listed in SEQ ID Nos. 1-120,855, and wherein each probe in the plurality of nucleic acid probes consists of one of the sequences listed in SEQ ID Nos. 1-120,855;
detecting a hybridization pattern; and
analyzing the hybridization pattern to determine a relative expression level of at least 100 genes.
14. The method of claim 13 wherein the yeast sample is from Saccharomyces cerevisisae.
15. The method of claim 13 wherein the yeast sample is from Schizosaccharomyces pombe.