Homogeneous populations of molecules
View Patent ↗The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
1. A method for estimating or determining an apparent or actual molecular weight of an uncharacterized molecule using a molecular weight standard set comprising at least two protein standards of known and different molecular weights, the method comprising:
a) separating by electrophoresis the uncharacterized molecule and at least one protein standard of the molecular weight standard set;
b) detecting the separated uncharacterized molecule and at least one separated protein standard of the molecular standard set; and
c) comparing at least one separated protein standard of the molecular standard set to the separated uncharacterized molecule to estimate or determine the apparent or actual molecular weight of the uncharacterized molecule;
wherein at least one of the at least two protein standards is a high molecular weight protein standard having an apparent molecular weight of at least 250 kDa by SDS PAGE,
wherein the high molecular weight protein standard is at least a partially homogeneous population of an isolated laminin polypeptide,
wherein at least one post-translational modification has been removed from the isolated laminin polypeptide to produce the at least partially homogenous population of the isolated laminin polypeptide, and
wherein the post-translational modification is selected from the group consisting of glycosylation and phosphorylation.
2. The method of claim 1 , wherein at least two of the protein standards of the set are at least partially homogeneous populations of laminin polypeptides, wherein at least one post-translational modification that has been removed from the laminin polypeptides, wherein the post-translational modification is selected from the group consisting of glycosylation and phosphorylation.